Somatic embryogenesis and plant regeneration in Azadirachta indica A. juss

Media for induction of somatic embryogenesis from immature cotyledonary tissues of Azadirachta indica (Neem) were determined. Callus was initiated on Murashige and Skoog medium supplemented with$0.5 mg \cdot liter^{-1}$of indol-3 acetic acid,$1.0 mg \cdot liter^{-1}$of 6-benzyl amino purine, and$100...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 1993-01, Vol.29P (1), p.38-42
Main Authors: Shrikhande, M, Thengane, S.R, Mascarenhas, A.F
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
AZADIRACHTA INDICA
Biological and medical sciences
Callus
COTILEDONES
COTYLEDON
COTYLEDONS
CULTIVO IN VITRO
CULTURE IN VITRO
CULTURE MEDIA
Embryos
Forest trees
Fundamental and applied biological sciences. Psychology
Germination
GLUCOSA
GLUCOSE
Histology
IN VITRO CULTURE
IN VITRO REGENERATION
Indian culture
MEDIO DE CULTIVO
Micropropagation
MILIEU DE CULTURE
Plant physiology and development
Plant tissues
Plantlets
Plants
REGENERACION IN VITRO
REGENERATION IN VITRO
REGENERATIVE ABILITY
Somatic embryogenesis
Tissue cultures, protoplasts
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Summary:Media for induction of somatic embryogenesis from immature cotyledonary tissues of Azadirachta indica (Neem) were determined. Callus was initiated on Murashige and Skoog medium supplemented with$0.5 mg \cdot liter^{-1}$of indol-3 acetic acid,$1.0 mg \cdot liter^{-1}$of 6-benzyl amino purine, and$1000 mg \cdot liter^{-1}$of casein hydrolysate. Effect of kinetin was also studied for embryo induction. Carbohydrate source in the form of sucrose and glucose alone and in combination was tested for embryogenic efficiency. Seventy percent embryos showed germination. Healthy plants were potted in sand and soil. Histologic studies confirmed indirect somatic embryogenesis.
ISSN:1054-5476
1475-2689
DOI:10.1007/BF02632237