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Cell Growth and λ Phage Development Controlled by the Same Essential Escherichia coli Gene, ftsH/hflB
The λ phage choice between lysis and lysogeny is influenced by certain host functions in Escherichia coli. We found that the frequency of λ lysogenization is markedly increased in the ftsH1 temperature-sensitive mutant. The ftsH gene, previously shown to code for an essential inner membrane protein...
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Published in: | Proceedings of the National Academy of Sciences - PNAS 1993-11, Vol.90 (22), p.10861-10865 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | The λ phage choice between lysis and lysogeny is influenced by certain host functions in Escherichia coli. We found that the frequency of λ lysogenization is markedly increased in the ftsH1 temperature-sensitive mutant. The ftsH gene, previously shown to code for an essential inner membrane protein with putative ATPase activity, is identical to hflB, a gene involved in the stability of the phage cII activator protein. The lysogenic decision controlled by FtsH/HflB is independent of that controlled by the protease HflA. Overproduction of FtsH/HflB suppresses the high frequency of lysogenization in an hflA null mutant. The FtsH/HflB protein, which stimulates cII degradation, may be a component of an HflA-independent proteolytic pathway, or it may act as a chaperone, maintaining cII in a conformation subject to proteolysis via such a pathway. Suppressor mutations of ftsH1 temperature-sensitive lethality, located in the fur gene (coding for the ferric uptake regulator), did not restore FtsH/HflB activity with respect to λ lysogenization. |
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ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.90.22.10861 |