Kinetics of appearance and disappearance of classes of bacterial ice nuclei support an aggregation model for ice nucleus assembly

The kinetics of appearance and disappearance of three classes of ice nuclei in Pseudomonas syringae was investigated under conditions where high-level expression of the ice nucleation phenotype was obtained. The appearance of types 1, 2, and 3 ice nuclei, catalyzing nucleation at -2 to -5, -5 to -7,...

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Bibliographic Details
Published in:Journal of Bacteriology 1993-11, Vol.175 (22), p.7216-7221
Main Authors: Ruggles, J.A, Nemecek-Marshall, M, Fall, R
Format: Article
Language:English
Subjects:
Bacteriology
Biochemistry
Biological and medical sciences
Biotechnology
Cell Membrane - physiology
Cell Membrane - ultrastructure
CONGELACION
CONGELATION
DANOS POR LA HELADA
DEGAT DU AU GEL
Fundamental and applied biological sciences. Psychology
Ice
Kinetics
Microbiology
Models, Biological
MUTANT
MUTANTES
Pseudomonas - growth & development
Pseudomonas - physiology
Pseudomonas - ultrastructure
PSEUDOMONAS SYRINGAE
TEMPERATURA AMBIENTE
Temperature
TEMPERATURE AMBIANTE
Time Factors
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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Summary:The kinetics of appearance and disappearance of three classes of ice nuclei in Pseudomonas syringae was investigated under conditions where high-level expression of the ice nucleation phenotype was obtained. The appearance of types 1, 2, and 3 ice nuclei, catalyzing nucleation at -2 to -5, -5 to -7, and -7 to -10 degrees C, respectively, was investigated during low-temperature induction in wild-type strains and in a unique, detergent-sensitive mutant that contained no type 3 ice nuclei when grown at 32 degrees C. Nuclei appeared in the following order: type 3, then type 2 and type 1. The disappearance of classes of ice nuclei was monitored during high-temperature treatment of fully induced cells; nuclei disappeared in the order type 1, type 2, and type 3. Although analysis of nucleation events is complicated by masking and unmasking of ice sites in the same cell, these temporal sequences of ice nucleus appearance or disappearance are consistent with an aggregation model for ice nucleus assembly (A. G. Govindarajan and S. E. Lindow, Proc. Natl. Acad. Sci. USA 85:1334-1338,1988; G. Warren and P. Wolber, Mol. Microbiol. 5:239-243, 1991)
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.175.22.7216-7221.1993