Family of glycosyl transferases needed for the synthesis of succinoglycan by Rhizobium meliloti

Rhizobium meliloti produces an acidic exopolysaccharide, termed succinoglycan or EPS I, that is important for invasion of the nodules that it elicits on its host, Medicago sativa. Succinoglycan is a high-molecular-weight polymer composed of repeating octasaccharide subunits. These subunits are synth...

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Bibliographic Details
Published in:Journal of Bacteriology 1993-11, Vol.175 (21), p.7033-7044
Main Authors: Glucksmann, M.A, Reuber, T.L, Walker, G.C
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Amino Acid Sequence
Bacteriology
Base Sequence
Biological and medical sciences
Blotting, Southern
Carbohydrate Sequence
Conserved Sequence
DNA, Bacterial - chemistry
DNA, Bacterial - metabolism
Flowers & plants
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes, Bacterial
Genetics
Genotype
GLICOSILTRANSFERASAS
GLYCOSYLTRANSFERASE
Hexosyltransferases - biosynthesis
Hexosyltransferases - genetics
Hexosyltransferases - metabolism
METABOLISME DES GLUCIDES
METABOLISMO DE CARBOHIDRATOS
Microbiology
Molecular Sequence Data
Multigene Family
Open Reading Frames
Plasmids
POLISACARIDOS
POLYHOLOSIDE
Polysaccharides, Bacterial - biosynthesis
RHIZOBIUM MELILOTI
SECUENCIA NUCLEICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEIQUE
Sinorhizobium meliloti - enzymology
Sinorhizobium meliloti - genetics
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Summary:Rhizobium meliloti produces an acidic exopolysaccharide, termed succinoglycan or EPS I, that is important for invasion of the nodules that it elicits on its host, Medicago sativa. Succinoglycan is a high-molecular-weight polymer composed of repeating octasaccharide subunits. These subunits are synthesized on membrane-bound isoprenoid lipid carriers, beginning with a galactose residue followed by seven glucose residues, and modified by the addition of acetate, succinate, and pyruvate. Biochemical characterizations of lipid-linked succinoglycan biosynthetic intermediates from previously identified exo mutant strains have been carried out in our laboratory (T. L. Reuber and G. C. Walker, Cell 74:269-280,.1993) to determine where each mutation blocks the biosynthetic pathway. We have carried out a fine structure genetic analysis of a portion of the cluster of exo genes present on the second symbiotic megaplasmid of R. meliloti and have identified several new genes. In addition, the DNA sequence of 16 kb of the exo cluster was determined and the genetic map was correlated with the DNA sequence. In this paper we present the sequence of a family of glycosyl transferases required for the synthesis of succinoglycan and discuss their functions
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.175.21.7033-7044.1993