Evaluation of an HPLC method for the determination of phylloquinone (vitamin K1) in various food matrices

The amount of phylloquinone (vitamin K1) in five different foods was determined by applying a highly sensitive HPLC method that incorporates postcolumn chemical reduction of the quinone followed by fluorescence detection of the hydroquinone form of the vitamin. After initial extraction in 2-propanol...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1994-02, Vol.42 (2), p.295-300
Main Authors: Booth, S.L, Davidson, K.W, Sadowski, J.A
Format: Article
Language:English
Subjects:
ALIMENTOS
Biological and medical sciences
CARNE DE RES
CHROMATOGRAPHIE LIQUIDE HAUTE PRESS
CROMATOGRAFIA LIQUIDA ALTA PRESION
EPINARD
ESPINACA
FLUORESCENCE
FLUORESCENCIA
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
JUGO DE LEGUMBRES
JUS DE LEGUME
LAIT
LECHE
METHODE D'ESSAI
Methods of analysis, processing and quality control, regulation, standards
METODOS DE ENSAYO
PAIN
PAN
PRODUIT ALIMENTAIRE
PURIFICACION
PURIFICATION
VIANDE BOVINE
VITAMINA K
VITAMINE K
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Summary:The amount of phylloquinone (vitamin K1) in five different foods was determined by applying a highly sensitive HPLC method that incorporates postcolumn chemical reduction of the quinone followed by fluorescence detection of the hydroquinone form of the vitamin. After initial extraction in 2-propanol and hexane, the food extracts were purified by solid-phase extraction (SPE) on silica gel and analyzed by HPLC or, depending on the matrix, further purified using liquid-phase reductive extraction or reversed-phase SPE on C18. Intra- and interday precision of the assay ranged from 6.6 to 13.6%. The between sample coefficient of variation in the phylloquinone content of 10 random bulk samples ranged from 7.8 to 44.6%, depending on the food item analyzed. In addition, endogenous dihydrophylloquinone was identified in foods containing hydrogenated oils, although its biological activity is unknown
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00038a013