Further evidence of a role for abscisic acid in conversion of somatic embryos of Daucus carota

Somatic embryogenesis has been shown to be an imperfect recapitulation of stages involved to form embryos from vegetative tissues. Although abscisic acid has been implicated in normalizing development, studies that specifically investigate conversion (vegetative leaf initiation) in somatic embryos a...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 1994-04, Vol.30P (2), p.96-103
Main Authors: Nickle, T.C, Yeung, E.C
Format: Article
Language:English
Subjects:
ABA
ACIDE ABSCISSIQUE
ACIDO ABSCISICO
Agronomy. Soil science and plant productions
APICAL MERISTEMS
Biological and medical sciences
Carrots
Cell Biology
CULTIVO IN VITRO
CULTURE IN VITRO
Cytoplasm
DAUCUS CAROTA
Developmental biology
EMBRIONES VEGETALES
Embryogenesis
EMBRYON VEGETAL
Embryos
FEUILLE
FLURIDONA
FLURIDONE
Fundamental and applied biological sciences. Psychology
Germination
Histology
HOJAS
IN VITRO CULTURE
LEAVES
MERISTEMAS APICALES
MERISTEME APICAL
ORGANOGENESE
ORGANOGENESIS
PLANT EMBRYOS
Plant physiology and development
Plantlets
Plants
SOMATIC EMBRYOGENESIS
Somatic embryos
Tissue cultures, protoplasts
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Summary:Somatic embryogenesis has been shown to be an imperfect recapitulation of stages involved to form embryos from vegetative tissues. Although abscisic acid has been implicated in normalizing development, studies that specifically investigate conversion (vegetative leaf initiation) in somatic embryos are lacking. This report documents a follow-up of a study that implicated abscisic acid as a vital factor in allowing embryos of Daucus carota to progress to the plantlet stage. Abscisic acid was determined to enhance conversion at doses ranging from 1 to 50 µM. Younger embryo stages were more responsive to abscisic acid application with regards to plantlet recovery. Pulses of abscisic acid were shown to elicit more rapid response with younger embryo stages, indicating more plastic development. Fluridone, an abscisic acid synthesis inhibitor, was shown to dramatically reduce conversion, even at low doses (
ISSN:1054-5476
1475-2689
DOI:10.1007/BF02632136