Involvement of the cyclic AMP-responsive element binding protein in bovine leukemia virus expression in vivo

• Published in: Journal of Virology 1994-09, Vol.68 (9), p.5845-5853
• Main Authors: Adam, E, Kerkhofs, P, Mammerickx, M, Kettmann, R, Burny, A, Droogmans, L, Willems, L
• Format: Article
• Language: English
• Subjects: ADN; Animals; B-Lymphocytes - physiology; Base Sequence; Biological and medical sciences; Cattle; Cyclic AMP - physiology; Cyclic AMP Response Element-Binding Protein - metabolism; DNA-Binding Proteins - metabolism; Enhancer Elements, Genetic; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Viral; Genetics; GENOMAS; GENOME; Leukemia Virus, Bovine - genetics; Life sciences; LINFOCITOS; LYMPHOCYTE; Microbiology; Molecular Sequence Data; Médecine vétérinaire & santé animale; Nuclear Proteins - metabolism; Oligodeoxyribonucleotides - metabolism; PROTEINAS; PROTEINE; Receptors, Cyclic AMP - physiology; Regulatory Sequences, Nucleic Acid; Repetitive Sequences, Nucleic Acid; RETROVIRIDAE; Sciences du vivant; Veterinary medicine & animal health; Virology; VIRUS DE LOS ANIMALES; VIRUS DES ANIMAUX
• ISSN: 0022-538X; 1098-5514; 1098-5514
• DOI: 10.1128/jvi.68.9.5845-5853.1994

The TAR element (Tax-responsive element; also called TxRE) is a major determinant of the regulation of bovine leukemia virus (BLT) expression. In order to gain insight into the mechanisms of viral expression, complexes formed between proteins and the TAR enhancer DNA were analyzed by gel retardation assays. We report here that nuclear lysates from ex vivo-isolated B lymphocytes contain proteins that specifically bind to TAR. An antibody directed toward the cyclic AMP-responsive element binding (CREB) protein supershifted a complex (C1) present only in BLT-infected B lymphocytes. The CREB protein thus appears to be a major transcription factor involved in BLT expression in vivo