Quantitation of Cinnamaldehyde and Cinnamic Acid in Blood by HPLC

A rapid and sensitive high performance liquid chromatographic (HPLC) method is described for the quantitatlon of cinnamaldehyde (CNMA) in rat blood at concentrations of 0.1–100 µg/mL. One of the metabolites of CNMA, cinnamic acid, can also be quantified simultaneously. CNMA is unstable in rat blood,...

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Bibliographic Details
Published in:Journal of analytical toxicology 1992-11, Vol.16 (6), p.359-362
Main Authors: Yuan, Jinhua, Bucher, John R., Goehl, Thomas J., Dieter, Mike P., Jameson, C.W.
Format: Article
Language:English
Subjects:
Acrolein - analogs & derivatives
Acrolein - blood
amines
Animals
Biological and medical sciences
Blood
Catalysis
Chromatography, High Pressure Liquid
cinnamaldehyde
Cinnamates - blood
Cinnamic acid
Drug Stability
Food toxicology
High-performance liquid chromatography
Kinetics
Male
Medical sciences
Metabolites
Oxidation
Quantitation
Rats
Temperature effects
Toxicology
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Description
Summary:A rapid and sensitive high performance liquid chromatographic (HPLC) method is described for the quantitatlon of cinnamaldehyde (CNMA) in rat blood at concentrations of 0.1–100 µg/mL. One of the metabolites of CNMA, cinnamic acid, can also be quantified simultaneously. CNMA is unstable in rat blood, probably because of rapid oxidation to cinnamic acid by enzymatic catalysis and nonenzymatic Schiff base formation with free amine groups of blood proteins. The disappearance of CNMA from rat blood follows first-order reaction kinetics with a half-life of 9 min at room temperature. The current analysis method involves the addition of an agent that will prevent CNMA degradation by denaturing protein and competitively blocking nucleophilic addition reactions, resulting in the nearly complete recovery of CNMA from blood. Recovery of cinnamic acid was approximately 80% at concentrations of 1–10 µg/mL.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/16.6.359