Calcium‐induced degradation of the Inositol (1,4,5)‐trisphosphate receptor/Ca2+‐channel

Ca2+‐induced degradation of the neuronal inositol (1,4,5)‐trisphosphate receptor, a protein which regulates Ca2+‐release from intracellular stores, has been examined. The IP3‐receptor, immunopurified from rat cerebellum, appeared to be an excellent substrate for purified Ca2+‐activated neutral prote...

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Bibliographic Details
Published in:FEBS letters 1993-06, Vol.323 (3), p.229-232
Main Authors: Magnusson, Andres, Haug, Lise Sofie, Walaas, S.Ivar, Østvold, Anne Carme
Format: Article
Language:English
Subjects:
Biological and medical sciences
Ca2+-channel
Calpain
Cell receptors
Cell structures and functions
Cerebellum
Fundamental and applied biological sciences. Psychology
IP3-receptor
Miscellaneous
Molecular and cellular biology
Signal transduction
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Summary:Ca2+‐induced degradation of the neuronal inositol (1,4,5)‐trisphosphate receptor, a protein which regulates Ca2+‐release from intracellular stores, has been examined. The IP3‐receptor, immunopurified from rat cerebellum, appeared to be an excellent substrate for purified Ca2+‐activated neutral protease (calpain). Incubation of membranes or immunopurified IP3‐receptor with Ca2+ and cerebellar cytosol also resulted in degradation of the receptor. Two main fragments with approximate molecular masses of 130 and 95 kDa were generated, both of which appeared to derive from the carboxyterminal Ca2+‐channel‐containing part of the protein. These data suggest that activation of the IP3‐receptor, by causing increases in intracellular [Ca2+], might result in degradation of the N‐terminal, IP3‐binding part of the receptor.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(93)81345-Z