The Protooncogene c-sea Encodes a Transmembrane Protein-Tyrosine Kinase Related to the Met/Hepatocyte Growth Factor/Scatter Factor Receptor

c-sea is the cellular homologue of the avian erythroblastosis virus S13-encoded oncogene v-sea. We have isolated and determined the nucleotide sequence of overlapping chicken cDNAs that encode the putative c-sea protooncogene product. The predicted reading frame encoded a 1404-aa polypeptide that ha...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1993-07, Vol.90 (13), p.6140-6144
Main Authors: Huff, Janice L., Jelinek, Mary Anne, Borgman, Cheryl A., Lansing, Timothy J., Parsons, J. Thomas
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Avian Proteins
Base Sequence
Biological and medical sciences
Cells
Cells, Cultured
Cellular biology
Chick Embryo
Chickens
Complementary DNA
Deoxyribonucleic acid
DNA
Fundamental and applied biological sciences. Psychology
Genes. Genome
Hepatocyte Growth Factor - metabolism
Hepatocytes
Leukocytes
Messenger RNA
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Oncogenes
Poultry
Protein-Tyrosine Kinases - chemistry
Protein-Tyrosine Kinases - genetics
Protein-Tyrosine Kinases - physiology
Proto-Oncogene Proteins - chemistry
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-met
Proto-Oncogenes
Receptors
Receptors, Cell Surface - genetics
Ribonucleic acid
RNA
RNA, Messenger - analysis
Viruses
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Summary:c-sea is the cellular homologue of the avian erythroblastosis virus S13-encoded oncogene v-sea. We have isolated and determined the nucleotide sequence of overlapping chicken cDNAs that encode the putative c-sea protooncogene product. The predicted reading frame encoded a 1404-aa polypeptide that had the structure of a receptor-like protein-tyrosine kinase and exhibited the highest degree of sequence similarity with the Met/hepatocyte growth factor/scatter factor receptor. Analysis of steady-state RNA expression revealed that c-sea mRNA levels were elevated ≈5-fold in chicken embryo cells transformed by activated variants of the src nonreceptor protein-tyrosine kinase gene but not in cells transformed by the nuclear oncogenes v-myc or v-rel. A survey of c-sea expression in a variety of chicken tissues indicated that the highest levels of mRNA were located in peripheral white blood cell populations and in the intestine.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.90.13.6140