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Specific pseudorabies virus infection of the rat visual system requires both gI and gp63 glycoproteins
Transneuronal transport of pseudorabies virus (PRV) from the retina to visual centers that mediate visual discrimination and reflexes requires specific genes in the unique short region of the PRV genome. In contrast, these same viral genes are not required to infect retinorecipient areas of the brai...
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| Published in: | Journal of Virology 1993-07, Vol.67 (7), p.3786-3797 |
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| Main Authors: | , , , , , |
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| container_end_page | 3797 |
| container_issue | 7 |
| container_start_page | 3786 |
| container_title | Journal of Virology |
| container_volume | 67 |
| creator | Whealy, M.E Card, J.P Robbins, A.K Dubin, J.R Rziha, H.J Enquist, L.W |
| description | Transneuronal transport of pseudorabies virus (PRV) from the retina to visual centers that mediate visual discrimination and reflexes requires specific genes in the unique short region of the PRV genome. In contrast, these same viral genes are not required to infect retinorecipient areas of the brain involved in circadian rhythm regulation. In this report, we demonstrate that viral mutants carrying defined deletions of the genes encoding glycoprotein gI or gp63, or both, result in the same dramatic transport defect. Efficient export of either gI or gp63 from the endoplasmic reticulum to the Golgi apparatus in a fibroblast cell line requires the presence of both proteins. We also show that gI and gp63 physically interact, as demonstrated by pulse-chase and sucrose gradient sedimentation experiments. Complex formation is rapid compared with homodimerization of PRV glycoprotein gII. We suggest that gI and gp63 function in concert to affect neurotropism in the rat visual circuitry and that a heterodimer is likely to be the unit of function |
| doi_str_mv | 10.1128/jvi.67.7.3786-3797.1993 |
| format | article |
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In contrast, these same viral genes are not required to infect retinorecipient areas of the brain involved in circadian rhythm regulation. In this report, we demonstrate that viral mutants carrying defined deletions of the genes encoding glycoprotein gI or gp63, or both, result in the same dramatic transport defect. Efficient export of either gI or gp63 from the endoplasmic reticulum to the Golgi apparatus in a fibroblast cell line requires the presence of both proteins. We also show that gI and gp63 physically interact, as demonstrated by pulse-chase and sucrose gradient sedimentation experiments. Complex formation is rapid compared with homodimerization of PRV glycoprotein gII. We suggest that gI and gp63 function in concert to affect neurotropism in the rat visual circuitry and that a heterodimer is likely to be the unit of function</description><identifier>ISSN: 0022-538X</identifier><identifier>EISSN: 1098-5514</identifier><identifier>DOI: 10.1128/jvi.67.7.3786-3797.1993</identifier><identifier>PMID: 8389905</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; APARATO GOLGI ; APPAREIL DE GOLGI ; Biological and medical sciences ; Cells, Cultured ; DNA Mutational Analysis ; Fundamental and applied biological sciences. Psychology ; GANGLION NERVEUX ; GANGLIOS ; Genes, Viral ; Genetics ; GLICOPROTEINAS ; GLYCOPROTEINE ; Herpesvirus 1, Suid - growth & development ; Herpesvirus 1, Suid - pathogenicity ; HERPETOVIRIDAE ; Hypothalamus - microbiology ; In Vitro Techniques ; Male ; METABOLISME DES PROTEINES ; METABOLISMO PROTEICO ; Microbiology ; MUTACION INDUCIDA ; MUTANT ; MUTANTES ; MUTATION PROVOQUEE ; NERF ; NERVIOS ; Neurons - microbiology ; OEIL ; OJOS ; PATHOGENESE ; PATOGENESIS ; PROTEINAS ; PROTEINE ; pseudorabies virus ; RAT ; RATA ; Rats ; Rats, Sprague-Dawley ; RETICULO ENDOPLASMATICO ; RETICULUM ENDOPLASMIQUE ; Retina - microbiology ; Swine ; TEJIDOS ANIMALES ; TISSU ANIMAL ; Viral Envelope Proteins - physiology ; Viral Structural Proteins - genetics ; Virology ; VIRUS DE LOS ANIMALES ; VIRUS DES ANIMAUX ; Virus Replication ; Visual Pathways - microbiology</subject><ispartof>Journal of Virology, 1993-07, Vol.67 (7), p.3786-3797</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c636t-8dff67ffa2d2cc89c719b12b480b0bfe0981f3e6077e795920d9db7dbbdfc9063</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC237743/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC237743/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4886447$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8389905$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Whealy, M.E</creatorcontrib><creatorcontrib>Card, J.P</creatorcontrib><creatorcontrib>Robbins, A.K</creatorcontrib><creatorcontrib>Dubin, J.R</creatorcontrib><creatorcontrib>Rziha, H.J</creatorcontrib><creatorcontrib>Enquist, L.W</creatorcontrib><title>Specific pseudorabies virus infection of the rat visual system requires both gI and gp63 glycoproteins</title><title>Journal of Virology</title><addtitle>J Virol</addtitle><description>Transneuronal transport of pseudorabies virus (PRV) from the retina to visual centers that mediate visual discrimination and reflexes requires specific genes in the unique short region of the PRV genome. In contrast, these same viral genes are not required to infect retinorecipient areas of the brain involved in circadian rhythm regulation. In this report, we demonstrate that viral mutants carrying defined deletions of the genes encoding glycoprotein gI or gp63, or both, result in the same dramatic transport defect. Efficient export of either gI or gp63 from the endoplasmic reticulum to the Golgi apparatus in a fibroblast cell line requires the presence of both proteins. We also show that gI and gp63 physically interact, as demonstrated by pulse-chase and sucrose gradient sedimentation experiments. Complex formation is rapid compared with homodimerization of PRV glycoprotein gII. We suggest that gI and gp63 function in concert to affect neurotropism in the rat visual circuitry and that a heterodimer is likely to be the unit of function</description><subject>Animals</subject><subject>APARATO GOLGI</subject><subject>APPAREIL DE GOLGI</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>DNA Mutational Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GANGLION NERVEUX</subject><subject>GANGLIOS</subject><subject>Genes, Viral</subject><subject>Genetics</subject><subject>GLICOPROTEINAS</subject><subject>GLYCOPROTEINE</subject><subject>Herpesvirus 1, Suid - growth & development</subject><subject>Herpesvirus 1, Suid - pathogenicity</subject><subject>HERPETOVIRIDAE</subject><subject>Hypothalamus - microbiology</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>METABOLISME DES PROTEINES</subject><subject>METABOLISMO PROTEICO</subject><subject>Microbiology</subject><subject>MUTACION INDUCIDA</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>MUTATION PROVOQUEE</subject><subject>NERF</subject><subject>NERVIOS</subject><subject>Neurons - microbiology</subject><subject>OEIL</subject><subject>OJOS</subject><subject>PATHOGENESE</subject><subject>PATOGENESIS</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>pseudorabies virus</subject><subject>RAT</subject><subject>RATA</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RETICULO ENDOPLASMATICO</subject><subject>RETICULUM ENDOPLASMIQUE</subject><subject>Retina - microbiology</subject><subject>Swine</subject><subject>TEJIDOS ANIMALES</subject><subject>TISSU ANIMAL</subject><subject>Viral Envelope Proteins - physiology</subject><subject>Viral Structural Proteins - genetics</subject><subject>Virology</subject><subject>VIRUS DE LOS ANIMALES</subject><subject>VIRUS DES ANIMAUX</subject><subject>Virus Replication</subject><subject>Visual Pathways - microbiology</subject><issn>0022-538X</issn><issn>1098-5514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNqFkUtr3DAURk1pSadp_0ChVIXSnV29rMciixL6CAS6SAPdCUmWbAWP5Uj2lPn31TDD0Ky60uI73726nKp6j2CDEBafH3ahYbzhDeGC1YRL3iApybNqg6AUddsi-rzaQIhx3RLx-2X1KucHCBGljF5UF4IIKWG7qfzd7GzwwYI5u7WLSZvgMtiFtGYQJu_sEuIEogfL4EDSS4nyqkeQ93lxW5Dc4xpSaZi4DKC_AXrqQD8zAvpxb-Oc4uLClF9XL7wes3tzei-r-29ff13_qG9_fr-5_nJbW0bYUovOe8a917jD1gppOZIGYUMFNNB4V05DnjgGOXdcthLDTnaGd8Z03krIyGV1dZw7r2brOuumJelRzSlsddqrqIN6mkxhUH3cKUw4p6T0P536KT6uLi9qG7J146gnF9eseMuZbCH6L4gYw0jitoD8CNoUc07Onz-DoDqoVEWlYlxxdVCpDirVQWVpvvv3lnPv5K7kH0-5zlaPPunJhnzGqBCMUl6wD0dsCP3wp7hSOm-fLi3M2yPjdVS6T2XM_Z2kuJVEkL-L1r8Z</recordid><startdate>19930701</startdate><enddate>19930701</enddate><creator>Whealy, M.E</creator><creator>Card, J.P</creator><creator>Robbins, A.K</creator><creator>Dubin, J.R</creator><creator>Rziha, H.J</creator><creator>Enquist, L.W</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19930701</creationdate><title>Specific pseudorabies virus infection of the rat visual system requires both gI and gp63 glycoproteins</title><author>Whealy, M.E ; Card, J.P ; Robbins, A.K ; Dubin, J.R ; Rziha, H.J ; Enquist, L.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c636t-8dff67ffa2d2cc89c719b12b480b0bfe0981f3e6077e795920d9db7dbbdfc9063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>APARATO GOLGI</topic><topic>APPAREIL DE GOLGI</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>DNA Mutational Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GANGLION NERVEUX</topic><topic>GANGLIOS</topic><topic>Genes, Viral</topic><topic>Genetics</topic><topic>GLICOPROTEINAS</topic><topic>GLYCOPROTEINE</topic><topic>Herpesvirus 1, Suid - growth & development</topic><topic>Herpesvirus 1, Suid - pathogenicity</topic><topic>HERPETOVIRIDAE</topic><topic>Hypothalamus - microbiology</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>METABOLISME DES PROTEINES</topic><topic>METABOLISMO PROTEICO</topic><topic>Microbiology</topic><topic>MUTACION INDUCIDA</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>MUTATION PROVOQUEE</topic><topic>NERF</topic><topic>NERVIOS</topic><topic>Neurons - microbiology</topic><topic>OEIL</topic><topic>OJOS</topic><topic>PATHOGENESE</topic><topic>PATOGENESIS</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>pseudorabies virus</topic><topic>RAT</topic><topic>RATA</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RETICULO ENDOPLASMATICO</topic><topic>RETICULUM ENDOPLASMIQUE</topic><topic>Retina - microbiology</topic><topic>Swine</topic><topic>TEJIDOS ANIMALES</topic><topic>TISSU ANIMAL</topic><topic>Viral Envelope Proteins - physiology</topic><topic>Viral Structural Proteins - genetics</topic><topic>Virology</topic><topic>VIRUS DE LOS ANIMALES</topic><topic>VIRUS DES ANIMAUX</topic><topic>Virus Replication</topic><topic>Visual Pathways - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Whealy, M.E</creatorcontrib><creatorcontrib>Card, J.P</creatorcontrib><creatorcontrib>Robbins, A.K</creatorcontrib><creatorcontrib>Dubin, J.R</creatorcontrib><creatorcontrib>Rziha, H.J</creatorcontrib><creatorcontrib>Enquist, L.W</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Whealy, M.E</au><au>Card, J.P</au><au>Robbins, A.K</au><au>Dubin, J.R</au><au>Rziha, H.J</au><au>Enquist, L.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific pseudorabies virus infection of the rat visual system requires both gI and gp63 glycoproteins</atitle><jtitle>Journal of Virology</jtitle><addtitle>J Virol</addtitle><date>1993-07-01</date><risdate>1993</risdate><volume>67</volume><issue>7</issue><spage>3786</spage><epage>3797</epage><pages>3786-3797</pages><issn>0022-538X</issn><eissn>1098-5514</eissn><abstract>Transneuronal transport of pseudorabies virus (PRV) from the retina to visual centers that mediate visual discrimination and reflexes requires specific genes in the unique short region of the PRV genome. In contrast, these same viral genes are not required to infect retinorecipient areas of the brain involved in circadian rhythm regulation. In this report, we demonstrate that viral mutants carrying defined deletions of the genes encoding glycoprotein gI or gp63, or both, result in the same dramatic transport defect. Efficient export of either gI or gp63 from the endoplasmic reticulum to the Golgi apparatus in a fibroblast cell line requires the presence of both proteins. We also show that gI and gp63 physically interact, as demonstrated by pulse-chase and sucrose gradient sedimentation experiments. Complex formation is rapid compared with homodimerization of PRV glycoprotein gII. We suggest that gI and gp63 function in concert to affect neurotropism in the rat visual circuitry and that a heterodimer is likely to be the unit of function</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>8389905</pmid><doi>10.1128/jvi.67.7.3786-3797.1993</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of Virology, 1993-07, Vol.67 (7), p.3786-3797 |
| issn | 0022-538X 1098-5514 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_4886447 |
| source | American Society for Microbiology; PubMed Central |
| subjects | Animals APARATO GOLGI APPAREIL DE GOLGI Biological and medical sciences Cells, Cultured DNA Mutational Analysis Fundamental and applied biological sciences. Psychology GANGLION NERVEUX GANGLIOS Genes, Viral Genetics GLICOPROTEINAS GLYCOPROTEINE Herpesvirus 1, Suid - growth & development Herpesvirus 1, Suid - pathogenicity HERPETOVIRIDAE Hypothalamus - microbiology In Vitro Techniques Male METABOLISME DES PROTEINES METABOLISMO PROTEICO Microbiology MUTACION INDUCIDA MUTANT MUTANTES MUTATION PROVOQUEE NERF NERVIOS Neurons - microbiology OEIL OJOS PATHOGENESE PATOGENESIS PROTEINAS PROTEINE pseudorabies virus RAT RATA Rats Rats, Sprague-Dawley RETICULO ENDOPLASMATICO RETICULUM ENDOPLASMIQUE Retina - microbiology Swine TEJIDOS ANIMALES TISSU ANIMAL Viral Envelope Proteins - physiology Viral Structural Proteins - genetics Virology VIRUS DE LOS ANIMALES VIRUS DES ANIMAUX Virus Replication Visual Pathways - microbiology |
| title | Specific pseudorabies virus infection of the rat visual system requires both gI and gp63 glycoproteins |
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