In vitro activity of the nitrogen fixation regulatory protein FIXJ from Rhizobium meliloti

Cell extracts of an Escherichia coli strain that overproduces the regulatory protein FIXJ from Rhizobium meliloti promoted transcription of fixK, a known FIXJ-dependent gene, in a coupled transcription-translation assay. Activation by FIXJ was dependent on the sigma 70 holoenzyme form of RNA polymer...

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Bibliographic Details
Published in:Journal of Bacteriology 1991-09, Vol.173 (18), p.5914-5917
Main Authors: Batut, J. (University of California, Berkeley, CA), Santero, E, Kustu, S
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacteriology
Base Sequence
Biological and medical sciences
DNA, Bacterial - genetics
DNA-Binding Proteins - genetics
ESCHERICHIA
FIJACION DEL NITROGENO
FIXATION DE L'AZOTE
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genetics
Life Sciences
Microbiology
Molecular Sequence Data
Nitrogen Fixation - genetics
NUCLEOTIDE
NUCLEOTIDOS
Oligonucleotides - chemistry
Promoter Regions, Genetic
PROTEINAS
PROTEINE
Rhizobium - genetics
RHIZOBIUM MELILOTI
Sigma Factor - genetics
Transcription, Genetic
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Summary:Cell extracts of an Escherichia coli strain that overproduces the regulatory protein FIXJ from Rhizobium meliloti promoted transcription of fixK, a known FIXJ-dependent gene, in a coupled transcription-translation assay. Activation by FIXJ was dependent on the sigma 70 holoenzyme form of RNA polymerase
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.173.18.5914-5917.1991