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Differential protein metabolism and gene expression in tomato [Lycopersicon esculentum] fruit during wounding stress
Changes in protein and RNA levels which occur in tomato (Lycopersicon esculentum cv. Pik-red) fruit tissue upon wounding were analyzed. Antibodies to nine plant proteins were used to identify the related cross-reactive proteins in tomato fruit tissue and quantify their abundance during wounding. Ant...
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Published in: | Plant and cell physiology 1991-10, Vol.32 (7), p.1057-1065 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | Changes in protein and RNA levels which occur in tomato (Lycopersicon esculentum cv. Pik-red) fruit tissue upon wounding were analyzed. Antibodies to nine plant proteins were used to identify the related cross-reactive proteins in tomato fruit tissue and quantify their abundance during wounding. Antibodies tested were pathogenesis-related proteins (PR-la, lb, lc; PR-2, N, O; PR-P, Q; basic chitinase), plastid proteins (sorbitol-6-phosphate dehydrogenase; acyl carrier protein; large subunit of Rubisco), cellulase and 33-kD cationic peroxidase. Marked differences in mRNA populations were also detected when the protein translation products following in vitro translation of poly(A)+ RNAs isolated from unwounded and wounded fruit tissue were compared. More direct evidence for the changes in mRNA levels was obtained by using specific cDNA probes, isolated from a cDNA library of wounded tomato pericarp tissue. Two cDNA clones identified RNAs that were induced upon wounding while a third cDNA clone identified a transcript whose abundance decreased. |
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ISSN: | 0032-0781 1471-9053 |
DOI: | 10.1093/oxfordjournals.pcp.a078169 |