Genotype relationships in Microseris elegans (Asteraceae, Lactuceae) revealed by DNA amplification from arbitrary primers (RAPDs)

The genetic relationships among 10 inbred lines representing 10 populations of the autogamous annual Microseris elegans from throughout California has been determined using random amplified polymorphic DNAs (RAPDs). Seventeen arbitrary 10 base pair primers produced 134 amplification products; 81 of...

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Bibliographic Details
Published in:Plant systematics and evolution 1992-01, Vol.179 (3/4), p.221-233
Main Authors: Heusden, A.W. van, Bachmann, K
Format: Article
Language:English
Subjects:
Asteraceae
Biological and medical sciences
DNA
DNA fingerprinting
Evolutionary genetics
Fundamental and applied biological sciences. Psychology
Gels
Genetic variation
genotype
Genotypes
geographical distribution
inbred lines
Inbred strains
isozymes
patterns
Phylogeny
Plant cytology, morphology, systematics, chorology and evolution
Plant evolution
Plants
population
Population distributions
Population genetics
random amplified polymorphic dna's
relationships
species
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Summary:The genetic relationships among 10 inbred lines representing 10 populations of the autogamous annual Microseris elegans from throughout California has been determined using random amplified polymorphic DNAs (RAPDs). Seventeen arbitrary 10 base pair primers produced 134 amplification products; 81 of these were shared by two or more strains. The 3 genotypes from Northern California are closely related as are 3 genotypes from Middle Californian populations which are not nearest neighbors. DNA fingerprinting with the oligonucleotide (GATA)4 gave compatible results, but the comparison was limited to samples run on one gel. Isoenzyme patterns are compatible with the DNA results, but limited by the very low number of informative polymorphisms. The clustered relationship among genotypes within a species and their geographic distribution suggests very restricted genetic recombination and an origin of new populations from randomly dispersed achenes within the range of the species.
ISSN:0378-2697
1615-6110
DOI:10.1007/bf00937598