Determination of the respiration kinetics for mycelial pellets of Phanerochaete chrysosporium

In mycelial pellet cultures of the white rot basidiomycete Phanerochaete chrysosporium, low oxygen concentration negatively affects the production of the extracellular lignin peroxidases and manganese peroxidases which are key components of the lignin-degrading system of this organism. To test the h...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1992-05, Vol.58 (5), p.1740-1745
Main Authors: Michel, F.C. Jr. (Michigan State University, East Lansing, MI), Grulke, E.A, Reddy, C.A
Format: Article
Language:English
Subjects:
09 BIOMASS FUELS
550800 > Morphology
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
BASIC BIOLOGICAL SCIENCES
Basidiomycota - cytology
Basidiomycota - enzymology
Basidiomycota - metabolism
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
CADENA RESPIRATORIA
CARBOHYDRATES
CELL CULTURES
Cellular biology
CHAINE RESPIRATOIRE
Fundamental and applied biological sciences. Psychology
FUNGI
Kinetics
LIGNIN
MATHEMATICAL MODELS
METABOLISM
MICELIO
Mission oriented research
MYCELIUM
ORGANIC COMPOUNDS
OXIGENO
Oxygen
Oxygen - metabolism
OXYGENE
PEROXIDASAS
PEROXYDASE
PHANEROCHAETE
Phanerochaete chrysosporium
Physiology and metabolism
PLANTS
POLYSACCHARIDES
RESPIRATION
SACCHARIDES 090900 > Biomass Fuels-- Processing-- (1990-)
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Summary:In mycelial pellet cultures of the white rot basidiomycete Phanerochaete chrysosporium, low oxygen concentration negatively affects the production of the extracellular lignin peroxidases and manganese peroxidases which are key components of the lignin-degrading system of this organism. To test the hypothesis that oxygen limitation in the pellets is responsible for this effect, oxygen microelectrodes were used to determine oxygen concentration gradients within the mycelial pellets of P. chrysosporium. Pellets were removed from oxygenated cultures, allowed to equilibrate with air, and probed with oxygen microelectrodes. The oxygen profiles were modelled assuming that O2 uptake follows a Michaelis-Menten relationship. The Vmax and Km values for oxygen uptake were 0.76 +/- 0.10 g/m3 of pellet per s and 0.5 +/- 0.3 g/m3, respectively. These kinetic values were used to predict respiration rates in air-flushed cultures, oxygen-flushed cultures, and cultures with large pellets (diameter 6 mm). The predicted respiration rates were independently validated by experimentally measuring the evolution of carbon dioxide from whole cultures
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.58.5.1740-1745.1992