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Determination of the respiration kinetics for mycelial pellets of Phanerochaete chrysosporium
In mycelial pellet cultures of the white rot basidiomycete Phanerochaete chrysosporium, low oxygen concentration negatively affects the production of the extracellular lignin peroxidases and manganese peroxidases which are key components of the lignin-degrading system of this organism. To test the h...
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| Published in: | Applied and Environmental Microbiology 1992-05, Vol.58 (5), p.1740-1745 |
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| container_end_page | 1745 |
| container_issue | 5 |
| container_start_page | 1740 |
| container_title | Applied and Environmental Microbiology |
| container_volume | 58 |
| creator | Michel, F.C. Jr. (Michigan State University, East Lansing, MI) Grulke, E.A Reddy, C.A |
| description | In mycelial pellet cultures of the white rot basidiomycete Phanerochaete chrysosporium, low oxygen concentration negatively affects the production of the extracellular lignin peroxidases and manganese peroxidases which are key components of the lignin-degrading system of this organism. To test the hypothesis that oxygen limitation in the pellets is responsible for this effect, oxygen microelectrodes were used to determine oxygen concentration gradients within the mycelial pellets of P. chrysosporium. Pellets were removed from oxygenated cultures, allowed to equilibrate with air, and probed with oxygen microelectrodes. The oxygen profiles were modelled assuming that O2 uptake follows a Michaelis-Menten relationship. The Vmax and Km values for oxygen uptake were 0.76 +/- 0.10 g/m3 of pellet per s and 0.5 +/- 0.3 g/m3, respectively. These kinetic values were used to predict respiration rates in air-flushed cultures, oxygen-flushed cultures, and cultures with large pellets (diameter 6 mm). The predicted respiration rates were independently validated by experimentally measuring the evolution of carbon dioxide from whole cultures |
| doi_str_mv | 10.1128/aem.58.5.1740-1745.1992 |
| format | article |
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Jr. (Michigan State University, East Lansing, MI) ; Grulke, E.A ; Reddy, C.A</creator><creatorcontrib>Michel, F.C. Jr. (Michigan State University, East Lansing, MI) ; Grulke, E.A ; Reddy, C.A</creatorcontrib><description>In mycelial pellet cultures of the white rot basidiomycete Phanerochaete chrysosporium, low oxygen concentration negatively affects the production of the extracellular lignin peroxidases and manganese peroxidases which are key components of the lignin-degrading system of this organism. To test the hypothesis that oxygen limitation in the pellets is responsible for this effect, oxygen microelectrodes were used to determine oxygen concentration gradients within the mycelial pellets of P. chrysosporium. Pellets were removed from oxygenated cultures, allowed to equilibrate with air, and probed with oxygen microelectrodes. The oxygen profiles were modelled assuming that O2 uptake follows a Michaelis-Menten relationship. The Vmax and Km values for oxygen uptake were 0.76 +/- 0.10 g/m3 of pellet per s and 0.5 +/- 0.3 g/m3, respectively. These kinetic values were used to predict respiration rates in air-flushed cultures, oxygen-flushed cultures, and cultures with large pellets (diameter 6 mm). The predicted respiration rates were independently validated by experimentally measuring the evolution of carbon dioxide from whole cultures</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.58.5.1740-1745.1992</identifier><identifier>PMID: 1622246</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>09 BIOMASS FUELS ; 550800 -- Morphology ; ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; BASIC BIOLOGICAL SCIENCES ; Basidiomycota - cytology ; Basidiomycota - enzymology ; Basidiomycota - metabolism ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; CADENA RESPIRATORIA ; CARBOHYDRATES ; CELL CULTURES ; Cellular biology ; CHAINE RESPIRATOIRE ; Fundamental and applied biological sciences. Psychology ; FUNGI ; Kinetics ; LIGNIN ; MATHEMATICAL MODELS ; METABOLISM ; MICELIO ; Mission oriented research ; MYCELIUM ; ORGANIC COMPOUNDS ; OXIGENO ; Oxygen ; Oxygen - metabolism ; OXYGENE ; PEROXIDASAS ; PEROXYDASE ; PHANEROCHAETE ; Phanerochaete chrysosporium ; Physiology and metabolism ; PLANTS ; POLYSACCHARIDES ; RESPIRATION ; SACCHARIDES 090900 -- Biomass Fuels-- Processing-- (1990-)</subject><ispartof>Applied and Environmental Microbiology, 1992-05, Vol.58 (5), p.1740-1745</ispartof><rights>1992 INIST-CNRS</rights><rights>Copyright American Society for Microbiology May 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c726t-144d0c2490b46bca389318df093273a831d59856645410996034cd1d9ac752003</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC195665/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC195665/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5331745$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1622246$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/7171528$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Michel, F.C. Jr. (Michigan State University, East Lansing, MI)</creatorcontrib><creatorcontrib>Grulke, E.A</creatorcontrib><creatorcontrib>Reddy, C.A</creatorcontrib><title>Determination of the respiration kinetics for mycelial pellets of Phanerochaete chrysosporium</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>In mycelial pellet cultures of the white rot basidiomycete Phanerochaete chrysosporium, low oxygen concentration negatively affects the production of the extracellular lignin peroxidases and manganese peroxidases which are key components of the lignin-degrading system of this organism. To test the hypothesis that oxygen limitation in the pellets is responsible for this effect, oxygen microelectrodes were used to determine oxygen concentration gradients within the mycelial pellets of P. chrysosporium. Pellets were removed from oxygenated cultures, allowed to equilibrate with air, and probed with oxygen microelectrodes. The oxygen profiles were modelled assuming that O2 uptake follows a Michaelis-Menten relationship. The Vmax and Km values for oxygen uptake were 0.76 +/- 0.10 g/m3 of pellet per s and 0.5 +/- 0.3 g/m3, respectively. These kinetic values were used to predict respiration rates in air-flushed cultures, oxygen-flushed cultures, and cultures with large pellets (diameter 6 mm). The predicted respiration rates were independently validated by experimentally measuring the evolution of carbon dioxide from whole cultures</description><subject>09 BIOMASS FUELS</subject><subject>550800 -- Morphology</subject><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Basidiomycota - cytology</subject><subject>Basidiomycota - enzymology</subject><subject>Basidiomycota - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>CADENA RESPIRATORIA</subject><subject>CARBOHYDRATES</subject><subject>CELL CULTURES</subject><subject>Cellular biology</subject><subject>CHAINE RESPIRATOIRE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>FUNGI</subject><subject>Kinetics</subject><subject>LIGNIN</subject><subject>MATHEMATICAL MODELS</subject><subject>METABOLISM</subject><subject>MICELIO</subject><subject>Mission oriented research</subject><subject>MYCELIUM</subject><subject>ORGANIC COMPOUNDS</subject><subject>OXIGENO</subject><subject>Oxygen</subject><subject>Oxygen - metabolism</subject><subject>OXYGENE</subject><subject>PEROXIDASAS</subject><subject>PEROXYDASE</subject><subject>PHANEROCHAETE</subject><subject>Phanerochaete chrysosporium</subject><subject>Physiology and metabolism</subject><subject>PLANTS</subject><subject>POLYSACCHARIDES</subject><subject>RESPIRATION</subject><subject>SACCHARIDES 090900 -- Biomass Fuels-- Processing-- (1990-)</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNqFUl2L1DAULaKs4-ofEMQq4lvHfDd58EHWT1hQ0H2UkEnTada26SapMv_eWzrMuk--JOHec87lnpyieI7RFmMi3xg3bLnc8i2uGarggJdS5F6xwUjJilMq7hcbhJSqCGHoYfEopWuEEENCnhVnWBAoi03x873LLg5-NNmHsQxtmTtXRpcmH9fSLz-67G0q2xDL4WBd701fTq7vXU4L4VtnRheD7QxIlbaLhxTSFKKfh8fFg9b0yT053ufF1ccPPy4-V5dfP325eHdZ2ZqIXGHGGmQJU2jHxM4aKhXFsmmRoqSmRlLccCW5EIwzWE8JRJltcKOMrTlBiJ4Xb1fdad4NrrFuzNH0eop-MPGgg_H6bmf0nd6H3xorUOXAf7nyQ8peJ-uzs50N4-hs1jWuMScSQK-PQ2K4mV3KevAJ7Ohh_TAnXVPEGOHiv0AwHylw_3bsCXgd5jiCU5ogrggldAHVK8jGkFJ07WktjPSSBQ1Z0FxqrpcsLAe8IAvAfPavK7e89fOh_-rYN8mavo1mtD6dYJChRQtgL1ZY5_fdHx-dNmm4OxQwT1dMa4I2-wgyV9_h94TCkv4FXBHQdg</recordid><startdate>19920501</startdate><enddate>19920501</enddate><creator>Michel, F.C. Jr. (Michigan State University, East Lansing, MI)</creator><creator>Grulke, E.A</creator><creator>Reddy, C.A</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19920501</creationdate><title>Determination of the respiration kinetics for mycelial pellets of Phanerochaete chrysosporium</title><author>Michel, F.C. Jr. (Michigan State University, East Lansing, MI) ; Grulke, E.A ; Reddy, C.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c726t-144d0c2490b46bca389318df093273a831d59856645410996034cd1d9ac752003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>09 BIOMASS FUELS</topic><topic>550800 -- Morphology</topic><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Basidiomycota - cytology</topic><topic>Basidiomycota - enzymology</topic><topic>Basidiomycota - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>CADENA RESPIRATORIA</topic><topic>CARBOHYDRATES</topic><topic>CELL CULTURES</topic><topic>Cellular biology</topic><topic>CHAINE RESPIRATOIRE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>FUNGI</topic><topic>Kinetics</topic><topic>LIGNIN</topic><topic>MATHEMATICAL MODELS</topic><topic>METABOLISM</topic><topic>MICELIO</topic><topic>Mission oriented research</topic><topic>MYCELIUM</topic><topic>ORGANIC COMPOUNDS</topic><topic>OXIGENO</topic><topic>Oxygen</topic><topic>Oxygen - metabolism</topic><topic>OXYGENE</topic><topic>PEROXIDASAS</topic><topic>PEROXYDASE</topic><topic>PHANEROCHAETE</topic><topic>Phanerochaete chrysosporium</topic><topic>Physiology and metabolism</topic><topic>PLANTS</topic><topic>POLYSACCHARIDES</topic><topic>RESPIRATION</topic><topic>SACCHARIDES 090900 -- Biomass Fuels-- Processing-- (1990-)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Michel, F.C. Jr. (Michigan State University, East Lansing, MI)</creatorcontrib><creatorcontrib>Grulke, E.A</creatorcontrib><creatorcontrib>Reddy, C.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Michel, F.C. Jr. (Michigan State University, East Lansing, MI)</au><au>Grulke, E.A</au><au>Reddy, C.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of the respiration kinetics for mycelial pellets of Phanerochaete chrysosporium</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1992-05-01</date><risdate>1992</risdate><volume>58</volume><issue>5</issue><spage>1740</spage><epage>1745</epage><pages>1740-1745</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>In mycelial pellet cultures of the white rot basidiomycete Phanerochaete chrysosporium, low oxygen concentration negatively affects the production of the extracellular lignin peroxidases and manganese peroxidases which are key components of the lignin-degrading system of this organism. To test the hypothesis that oxygen limitation in the pellets is responsible for this effect, oxygen microelectrodes were used to determine oxygen concentration gradients within the mycelial pellets of P. chrysosporium. Pellets were removed from oxygenated cultures, allowed to equilibrate with air, and probed with oxygen microelectrodes. The oxygen profiles were modelled assuming that O2 uptake follows a Michaelis-Menten relationship. The Vmax and Km values for oxygen uptake were 0.76 +/- 0.10 g/m3 of pellet per s and 0.5 +/- 0.3 g/m3, respectively. These kinetic values were used to predict respiration rates in air-flushed cultures, oxygen-flushed cultures, and cultures with large pellets (diameter 6 mm). The predicted respiration rates were independently validated by experimentally measuring the evolution of carbon dioxide from whole cultures</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>1622246</pmid><doi>10.1128/aem.58.5.1740-1745.1992</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied and Environmental Microbiology, 1992-05, Vol.58 (5), p.1740-1745 |
| issn | 0099-2240 1098-5336 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_5331745 |
| source | American Society for Microbiology Journals; PubMed Central |
| subjects | 09 BIOMASS FUELS 550800 -- Morphology ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE BASIC BIOLOGICAL SCIENCES Basidiomycota - cytology Basidiomycota - enzymology Basidiomycota - metabolism Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology CADENA RESPIRATORIA CARBOHYDRATES CELL CULTURES Cellular biology CHAINE RESPIRATOIRE Fundamental and applied biological sciences. Psychology FUNGI Kinetics LIGNIN MATHEMATICAL MODELS METABOLISM MICELIO Mission oriented research MYCELIUM ORGANIC COMPOUNDS OXIGENO Oxygen Oxygen - metabolism OXYGENE PEROXIDASAS PEROXYDASE PHANEROCHAETE Phanerochaete chrysosporium Physiology and metabolism PLANTS POLYSACCHARIDES RESPIRATION SACCHARIDES 090900 -- Biomass Fuels-- Processing-- (1990-) |
| title | Determination of the respiration kinetics for mycelial pellets of Phanerochaete chrysosporium |
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