Enhanced expression of a mosquito larvicidal gene(s) of Bacillus sphaericus 1593M in Escherichia coli

Bacillus sphaericus is known for its potential as a biocontrol agent to Culex and Anopheles species of mosquitoes. A 3.6-kb-DNA fragment of B. sphaericus 1593M encoding mosquito larvicidal activity has earlier been cloned by us in E. coli, using the vector pBR322 and the recombinant had a low larvic...

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Bibliographic Details
Published in:Current science (Bangalore) 1992-08, Vol.63 (4), p.205-208
Main Authors: Rajamohan, F., Suresh, G., Jayaraman, Kunthala
Format: Article
Language:English
Subjects:
Alkalies
Bacillus
Bacteriology
Biological and medical sciences
Crystals
Fundamental and applied biological sciences. Psychology
Genes
Genetic vectors
Larvae
Microbiology
Mosquitos
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Plasmids
RESEARCH COMMUNICATIONS
Toxicity
Toxins
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Summary:Bacillus sphaericus is known for its potential as a biocontrol agent to Culex and Anopheles species of mosquitoes. A 3.6-kb-DNA fragment of B. sphaericus 1593M encoding mosquito larvicidal activity has earlier been cloned by us in E. coli, using the vector pBR322 and the recombinant had a low larvicidal activity. This fragment has now been recloned into an expression vector of E. coli, pPR683. The resultant recombinant clone expressed high levels of larvicidal factors under the control of tac promoter at levels comparable to that of B. sphaericus 1593M. Since lactose can be an effective inducer of the larvicidal factors in this recombinant, an economic production of bioinsecticidal factors is forecasted.
ISSN:0011-3891