Morphogenic potential of cultured floral explants of Crocus sativus L. for the in vitro production of saffron

Explants of immature ovaries, stigmas, anthers and petals of Crocus sativus were cultured on White's media supplemented with either NAA and zeatin or 2,4-D and BAP in various combinations. The formation of stigma-like structures occurred on the explants or on the callus derived from the explant...

Full description

Saved in:
Bibliographic Details
Published in:Journal of experimental botany 1990, Vol.41 (222), p.47-52
Main Authors: Fakhrai, F, Evans, P.K
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Anthers
Biological and medical sciences
Biotechnology
Callus
Corms
corolla
Crocus sativus
crop production
Culture media
Economic plant physiology
Eukaryotic cell cultures
Flower stigma
Fundamental and applied biological sciences. Psychology
gynoecium
in vitro
In vitro culture
Methods. Procedures. Technologies
Miscellaneous
Ovaries
Petals
Pigments
Plant cells and fungal cells
Plant physiology and development
Saffron
stigma
tissue culture
Tissue culture techniques
Tissue cultures, protoplasts
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Explants of immature ovaries, stigmas, anthers and petals of Crocus sativus were cultured on White's media supplemented with either NAA and zeatin or 2,4-D and BAP in various combinations. The formation of stigma-like structures occurred on the explants or on the callus derived from the explants, but this was only observed when NAA and zeatin were used. Formation of stigma-like structures were observed on anthers, petals, stigmas and half-ovaries, with the best result being obtained on explants consisting of half-ovaries cultured on medium containing NAA at 4.0 mg dm-3 and zeatin at 4.0 mg dm-3. These stigmas developed an intense orange pigment and grew to 3.0 cm in length and had a strong saffron aroma. A preliminary comparison using thin layer chromatography of the yellow pigments produced by the stigma-like structures grown in vitro and those grown naturally showed the pigment composition to be similar.
ISSN:0022-0957
1460-2431