Characterization of Invasion Plasmid Antigen Genes (ipaBCD) from Shigella flexneri

The large invasion plasmid of Shigella flexneri M9OT-W was used to generate recombinant plasmids carrying the ipaA, -B, -C, and -D genes, whose products are associated with the entry of the bacteria into colonic epithelial cells. Complete DNA sequences of ipaB, -C, and -D were determined. The protei...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-12, Vol.85 (23), p.9317-9321
Main Authors: Venkatesan, Malabi M., Buysse, Jerry M., Kopecko, Dennis J.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antigens
Antigens, Bacterial - genetics
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Bacteriology
Base Sequence
Biological and medical sciences
Blotting, Northern
DNA
Epithelial cells
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Genes. Genome
Genetic hybridization
Genetics
Human bacterial diseases
Infectious diseases
Medical sciences
Microbiology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleic Acid Hybridization
Nucleotide sequences
Plasmids
Protein Conformation
Restriction Mapping
RNA
Shigella
Shigella flexneri - genetics
Shigella flexneri - immunology
Shigella flexneri - pathogenicity
Tablet computers
Tropical medicine
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Description
Summary:The large invasion plasmid of Shigella flexneri M9OT-W was used to generate recombinant plasmids carrying the ipaA, -B, -C, and -D genes, whose products are associated with the entry of the bacteria into colonic epithelial cells. Complete DNA sequences of ipaB, -C, and -D were determined. The proteins predicted (62, 42, and 37 kDa, respectively) from the nucleotide sequences lack a signalpeptide sequence. Hydrophilic segments of the IpaB and IpaC proteins were found to overlap known epitopic domains of these membrane antigens. Analysis of total RNA demonstrated that temperature control of ipa gene expression occurs at the level of transcription. Multiple mRNA bands were detected by using ipa gene fragments as hybridization probes, and a putative transcript map for the ipa genes was constructed. Comparison of this map with the DNA sequence reveals a complex system of ipa gene regulation.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.23.9317