Detection of kestoses and kestose-related oligosaccharides in extracts of Festuca arundinacea, Dactylis glomerata L., and Asparagus officinalis L. root cultures and invertase by 13C and 1H nuclear magnetic resonance spectroscopy

A previous study (KL Forsythe, MS Feather [1989] Carbohydr Res 185: 315-319) showed that 13C nuclear magnetic resonance spectroscopy can be used to detect and identify mixtures of 1-kestose and neokestose after conversion to the acetate derivatives. In this study, unequivocal assignments are made fo...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology (Bethesda) 1990-04, Vol.92 (4), p.1014-1020
Main Authors: Forsythe, K.L. (University of Missouri, Columbia, MO), Feather, M.S, Gracz, H, Wong, T.C
Format: Article
Language:English
Subjects:
Acetates
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ANALISIS
ANALYSE
Analytical, structural and metabolic biochemistry
ASPARAGUS
AZUCARES
Biological and medical sciences
Carbohydrates
COMPOSICION
COMPOSITION
CULTIVO DE CELULAS
CULTURE DE CELLULES
DACTYLIS GLOMERATA
FESTUCA ARUNDINACEA
Fructans
FRUCTOFURANOSIDASA
FRUCTOFURANOSIDASE
Fundamental and applied biological sciences. Psychology
Oligomers
Oligosaccharides
Other biological molecules
Plants
Protons
RACINE
RAICES
Spectroscopy
SUCRES
Tetrasaccharides
Trisaccharides
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A previous study (KL Forsythe, MS Feather [1989] Carbohydr Res 185: 315-319) showed that 13C nuclear magnetic resonance spectroscopy can be used to detect and identify mixtures of 1-kestose and neokestose after conversion to the acetate derivatives. In this study, unequivocal assignments are made for the anomeric carbon and proton signals for the above two trisaccharide acetates as well as for 6-kestose hendecaacetate and for nystose tetradecaacetate (a 1-kestose-derived tetrasaccharide). A number of oligosaccharide fractions were isolated from several plant species, converted to the acetates, and nuclear magnetic resonance spectra obtained. Using the above reference data, the following information was obtained. The trisaccharide fraction from Dactylis glomerata L. stem tissue and Asparagus officinalis L. roots contain both 1-kestose and neokestose, and the tetrasaccharide fractions contain three components, one of which is nystose. Penta- and hexasaccharide acetates were also isolated from A. officinalis L. roots and were found to contain, respectively, four and at least five components. All components of both of the above species appear to contain a kestose residue and to be produced by the sequential addition of fructofuranosyl units to these. The trisaccharide fraction from Festuca arundinacea is complex, and contains at least five different components, two of which appear to be 1-kestose and neokestose
ISSN:0032-0889
1532-2548