Biochemistry and molecular biology of competence of plant cell differentiation and regeneration in vitro—a review

• Published in: Current science (Bangalore) 1990-03, Vol.59 (6), p.308-311
• Main Authors: Sethi, Urmil, Guha-Mukherjee, Sipra
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Amino acids; Biochemistry; Biological and medical sciences; Cell cycle; Cell division; Cellular differentiation; Cultured cells; Economic plant physiology; Fundamental and applied biological sciences. Psychology; Hormones; In vitro culture; Plant cells; Plant physiology and development; Plant tissues; Polyamines; REVIEW ARTICLE; Tissue cultures, protoplasts
• ISSN: 0011-3891

In spite of accumulation of voluminous literature on induction of cell division and differentiation in tissue culture, little information is available about the biochemical and molecular events that control it. In Brassica oleracea, exogenous supply of hormones, polyamines and certain amino acids lead to vigorous proliferation, whereas addition of inhibitors to inhibit polyamine or ethylene biosynthesis, transcription and translation, or abscisic acid, results in differentiation. Red/far-red light also induces proliferation or differentiation. There are also evidences to suggest that the phosphoinositide intracellular signalling system to generate second messengers, well established in the animal kingdom, also exists in plants. Some constituents of this cycle have been identified. It appears that through control of the cell cycle by arresting cells at G1 or G2 phases, it is possible to ensure differentiation in plant tissue culture.