Identification of Frankia strains by direct DNA hybridization of crushed nodules

A hybridization procedure was developed to identify Frankia strains inside actinorhizae by direct probing of crushed root nodules. The probe consisted of an indigenous cryptic plasmid. This well-conserved, 8-kilobase plasmid was detected in Frankia isolates that were very close taxonomically (they p...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1988-10, Vol.54 (10), p.2500-2503
Main Authors: SIMONET, P, NGUYEN THI LE, TEISSIER DU CROS, E, BARDIN, R
Format: Article
Language:English
Subjects:
adn
Agronomy. Soil science and plant productions
alnus glutinosa
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
dna
Economic plant physiology
formation de nodosites
Frankia
Fundamental and applied biological sciences. Psychology
hibridacion
hybridation
hybridizing
identificacion
identification
Microbiology
Microorganism-Plant Interactions
nodulacion
root nodulation
Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
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Summary:A hybridization procedure was developed to identify Frankia strains inside actinorhizae by direct probing of crushed root nodules. The probe consisted of an indigenous cryptic plasmid. This well-conserved, 8-kilobase plasmid was detected in Frankia isolates that were very close taxonomically (they possessed a very high DNA sequence homology). The probe did not hybridize to the DNA of Frankia isolates which did not carry the plasmid. Endophyte DNA was extracted by a modification of a technique originally developed for the detection of plasmids in Frankia isolates. The hybridization procedure applied to nodules collected in a stand of alder permitted determination of a distribution map of the plasmid-bearing Frankia strains.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.54.10.2500-2503.1988