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Identification of Frankia strains by direct DNA hybridization of crushed nodules

A hybridization procedure was developed to identify Frankia strains inside actinorhizae by direct probing of crushed root nodules. The probe consisted of an indigenous cryptic plasmid. This well-conserved, 8-kilobase plasmid was detected in Frankia isolates that were very close taxonomically (they p...

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Published in:	Applied and Environmental Microbiology 1988-10, Vol.54 (10), p.2500-2503
Main Authors:	SIMONET, P, NGUYEN THI LE, TEISSIER DU CROS, E, BARDIN, R
Format:	Article
Language:	English
Subjects:	adn Agronomy. Soil science and plant productions alnus glutinosa Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences dna Economic plant physiology formation de nodosites Frankia Fundamental and applied biological sciences. Psychology hibridacion hybridation hybridizing identificacion identification Microbiology Microorganism-Plant Interactions nodulacion root nodulation Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
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cited_by	cdi_FETCH-LOGICAL-c581t-3b79d7ebb9f2d9ae87ff44c104cee7ad3180ad8d1971a6b78911eb00c471d1293
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container_title	Applied and Environmental Microbiology
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creator	SIMONET, P NGUYEN THI LE TEISSIER DU CROS, E BARDIN, R
description	A hybridization procedure was developed to identify Frankia strains inside actinorhizae by direct probing of crushed root nodules. The probe consisted of an indigenous cryptic plasmid. This well-conserved, 8-kilobase plasmid was detected in Frankia isolates that were very close taxonomically (they possessed a very high DNA sequence homology). The probe did not hybridize to the DNA of Frankia isolates which did not carry the plasmid. Endophyte DNA was extracted by a modification of a technique originally developed for the detection of plasmids in Frankia isolates. The hybridization procedure applied to nodules collected in a stand of alder permitted determination of a distribution map of the plasmid-bearing Frankia strains.
doi_str_mv	10.1128/aem.54.10.2500-2503.1988
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The probe consisted of an indigenous cryptic plasmid. This well-conserved, 8-kilobase plasmid was detected in Frankia isolates that were very close taxonomically (they possessed a very high DNA sequence homology). The probe did not hybridize to the DNA of Frankia isolates which did not carry the plasmid. Endophyte DNA was extracted by a modification of a technique originally developed for the detection of plasmids in Frankia isolates. The hybridization procedure applied to nodules collected in a stand of alder permitted determination of a distribution map of the plasmid-bearing Frankia strains.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.54.10.2500-2503.1988</identifier><identifier>PMID: 16347757</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>adn ; Agronomy. 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The probe consisted of an indigenous cryptic plasmid. This well-conserved, 8-kilobase plasmid was detected in Frankia isolates that were very close taxonomically (they possessed a very high DNA sequence homology). The probe did not hybridize to the DNA of Frankia isolates which did not carry the plasmid. Endophyte DNA was extracted by a modification of a technique originally developed for the detection of plasmids in Frankia isolates. The hybridization procedure applied to nodules collected in a stand of alder permitted determination of a distribution map of the plasmid-bearing Frankia strains.</description><subject>adn</subject><subject>Agronomy. Soil science and plant productions</subject><subject>alnus glutinosa</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>dna</subject><subject>Economic plant physiology</subject><subject>formation de nodosites</subject><subject>Frankia</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hibridacion</subject><subject>hybridation</subject><subject>hybridizing</subject><subject>identificacion</subject><subject>identification</subject><subject>Microbiology</subject><subject>Microorganism-Plant Interactions</subject><subject>nodulacion</subject><subject>root nodulation</subject><subject>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNp9kctu1DAUhi1ERYeBV0ARQrDK9PgW2wsWVaFQqSpI0LXl2M7EkEuxE9Dw9Dia0ZSyYOFjHZ3vPxf9CBUYNhgTeWZ8v-Fsk1PCAcoc6AYrKR-hFQYlS05p9RitAJQqCWFwip6m9A0AGFTyCTrFFWVCcLFCn6-cH6bQBGumMA7F2BSX0QzfgynSFE0YUlHvCheit1Px7ua8aHd1DC78PuI2zqn1rhhGN3c-PUMnjemSf3741-j28v3Xi4_l9acPVxfn16XlEk8lrYVywte1aohTxkvRNIxZDMx6L4yjWIJx0mElsKlqIRXGvgawTGCHiaJr9Hbf926ue-9sviKaTt_F0Ju406MJ-mFlCK3ejj81AUZUlfVvDvo4_ph9mnQfkvVdZwY_zkkLShkTlSSZfP1fEnNglOW3RnIP2jimFH1z3AaDXnzT2TfN2ZIuvi2B6sW3LH3x9zX3woNRGXh1AEyypmuyRzakIyeAc0Jpxl7usTZs21_ZNW1S_8_Y-2GNGbXZxtzn9kteQwFwIXKbPx_wtzE</recordid><startdate>19881001</startdate><enddate>19881001</enddate><creator>SIMONET, P</creator><creator>NGUYEN THI LE</creator><creator>TEISSIER DU CROS, E</creator><creator>BARDIN, R</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19881001</creationdate><title>Identification of Frankia strains by direct DNA hybridization of crushed nodules</title><author>SIMONET, P ; NGUYEN THI LE ; TEISSIER DU CROS, E ; BARDIN, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c581t-3b79d7ebb9f2d9ae87ff44c104cee7ad3180ad8d1971a6b78911eb00c471d1293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>adn</topic><topic>Agronomy. Soil science and plant productions</topic><topic>alnus glutinosa</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>dna</topic><topic>Economic plant physiology</topic><topic>formation de nodosites</topic><topic>Frankia</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hibridacion</topic><topic>hybridation</topic><topic>hybridizing</topic><topic>identificacion</topic><topic>identification</topic><topic>Microbiology</topic><topic>Microorganism-Plant Interactions</topic><topic>nodulacion</topic><topic>root nodulation</topic><topic>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SIMONET, P</creatorcontrib><creatorcontrib>NGUYEN THI LE</creatorcontrib><creatorcontrib>TEISSIER DU CROS, E</creatorcontrib><creatorcontrib>BARDIN, R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SIMONET, P</au><au>NGUYEN THI LE</au><au>TEISSIER DU CROS, E</au><au>BARDIN, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Frankia strains by direct DNA hybridization of crushed nodules</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1988-10-01</date><risdate>1988</risdate><volume>54</volume><issue>10</issue><spage>2500</spage><epage>2503</epage><pages>2500-2503</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>A hybridization procedure was developed to identify Frankia strains inside actinorhizae by direct probing of crushed root nodules. The probe consisted of an indigenous cryptic plasmid. This well-conserved, 8-kilobase plasmid was detected in Frankia isolates that were very close taxonomically (they possessed a very high DNA sequence homology). The probe did not hybridize to the DNA of Frankia isolates which did not carry the plasmid. Endophyte DNA was extracted by a modification of a technique originally developed for the detection of plasmids in Frankia isolates. The hybridization procedure applied to nodules collected in a stand of alder permitted determination of a distribution map of the plasmid-bearing Frankia strains.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16347757</pmid><doi>10.1128/aem.54.10.2500-2503.1988</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
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source	American Society for Microbiology Journals; PubMed Central
subjects	adn Agronomy. Soil science and plant productions alnus glutinosa Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences dna Economic plant physiology formation de nodosites Frankia Fundamental and applied biological sciences. Psychology hibridacion hybridation hybridizing identificacion identification Microbiology Microorganism-Plant Interactions nodulacion root nodulation Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
title	Identification of Frankia strains by direct DNA hybridization of crushed nodules
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