Human and Rat Mast Cell High-Affinity Immunoglobulin E Receptors: Characterization of Putative α -Chain Gene Products

• Published in: Proceedings of the National Academy of Sciences - PNAS 1988-03, Vol.85 (6), p.1907-1911
• Main Authors: Shimizu, Akira, Tepler, Isidore, Benfey, Philip N., Berenstein, Elsa H., Siraganjan, Reuben P., Leder, Philip
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Amino acids; ANIMAL CELLS; ANIMALS; Base Sequence; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; Biological and medical sciences; Cell lines; CHEMICAL REACTIONS; CHROMATOGRAPHY; Cloning, Molecular; Complementary DNA; CONNECTIVE TISSUE CELLS; CROSS-LINKING; DAYS LIVING RADIOISOTOPES; DNA; DNA - metabolism; DNA SEQUENCING; ELECTRON CAPTURE RADIOISOTOPES; Fundamental and applied biological sciences. Psychology; Fundamental immunology; GENES; GLOBULINS; Humans; HYBRIDIZATION; IgE receptors; Immediate hypersensitivity. Allergy. Anaphylaxis, etc; Immunobiology; IMMUNOGLOBULINS; INTERMEDIATE MASS NUCLEI; IODINE 125; IODINE ISOTOPES; ISOTOPES; LABELLED COMPOUNDS; LIQUID COLUMN CHROMATOGRAPHY; MAMMALS; MAN; MAST CELLS; Mast Cells - metabolism; MEMBRANE PROTEINS; Messenger RNA; Molecular Sequence Data; NUCLEI; NUCLEIC ACIDS; Nucleotide sequences; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; Peptide Mapping; POLYMERIZATION; PRIMATES; PROTEINS; PURIFICATION; RADIOISOTOPES; RATS; Reaction mechanisms, antibodies, chemical mediators; RECEPTORS; Receptors, Fc - metabolism; Receptors, IgE; RECOMBINANT DNA; RNA; RODENTS; SEPARATION PROCESSES; Sequencing; SOMATIC CELLS; STRUCTURAL CHEMICAL ANALYSIS; TRANSCRIPTION; VERTEBRATES 550201 > Biochemistry-- Tracer Techniques
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.85.6.1907

We have cloned and determined the entire nucleotide sequence of cDNAs corresponding to the putative α subunits of the human and rat mast cell high-affinity IgE receptors. Both human and rat cDNAs encode an NH2-terminal signal peptide, two immunoglobulin-like extracellular domains (encoded by discrete exons), a hydrophobic transmembrane region, and a positively charged cytoplasmic tail. The human and rat α subunits share an overall homology with one another and the immunoglobulin gene family, suggesting that they arose from a common ancestral gene and continue to share structural homology with their ligands. In addition, the rat gene is transcribed into at least three distinct forms, each of which yields a somewhat different coding sequence.