Measurement of alkaline phosphatase activity by electrochemical detection of phosphate esters: Application to amperometric enzyme immunoassay

Dc cyclic voltammetry and chronoamperometry have been used to examine the electrochemistry of a series of phosphate esters that are substrates for calf intestinal alkaline phosphatase. p-Aminophenyl phosphate was selected as the most suitable substrate for the electrochemical measurement of alkaline...

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Bibliographic Details
Published in:Journal of electroanalytical chemistry and interfacial electrochemistry 1989-07, Vol.266 (2), p.309-316
Main Authors: Frew, Jane E., Foulds, Nicola C., Wilshere, Jane M., Forrow, Nigel J., Green, Monika J.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Hydrolases
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Summary:Dc cyclic voltammetry and chronoamperometry have been used to examine the electrochemistry of a series of phosphate esters that are substrates for calf intestinal alkaline phosphatase. p-Aminophenyl phosphate was selected as the most suitable substrate for the electrochemical measurement of alkaline phosphatase activity. Picomolar levels of enzyme can be detected, which enables the design of electrochemical immunoassays using this system. Theophylline and phenytoin have been used as model analytes in competition and displacement assay formats.
ISSN:0022-0728
DOI:10.1016/0022-0728(89)85077-6