Humoral Immune Response to the Entire Human Immunodeficiency Virus Envelope Glycoprotein Made in Insect Cells

The human immunodeficiency virus envelope gene was expressed in insect cells by using a Baculovirus expression vector. The protein has an apparent molecular mass of 160 kDa, appears on the surface of infected insect cells, and does not appear to be cleaved to glycoproteins gp 120 and gp41. Goats imm...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-10, Vol.84 (19), p.6924-6928
Main Authors: Rusche, James R., Lynn, Debra L., Robert-Guroff, Marjorie, Langlois, Alphonse J., Lyerly, H. Kim, Carson, Helen, Krohn, Kai, Ranki, Annamari, Gallo, Robert C., Bolognesi, Dani P., Putney, Scott D., Matthews, Thomas J.
Format: Article
Language:English
Subjects:
AIDS/HIV
Animals
Antibody Formation
Antiserum
Baculoviridae
baculovirus
Biological and medical sciences
Biotechnology
Cell Line
Cell lines
cloning vectors
envelopes
Fundamental and applied biological sciences. Psychology
Gels
Genes
Genes, Viral
Genetic engineering
Genetic technics
Genetic Vectors
glycoproteins
Goats - immunology
HIV
HIV - genetics
HIV - immunology
human immunodeficiency virus
immune response (humoral)
Infections
Insect genetics
Insect vectors
Insect Viruses - genetics
Methods. Procedures. Technologies
Microbiology
Molecular cloning
Moths
Recombinant Proteins - immunology
Spodoptera frugiperda
vaccination
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Viral Envelope Proteins - immunology
Virology
Viruses
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Description
Summary:The human immunodeficiency virus envelope gene was expressed in insect cells by using a Baculovirus expression vector. The protein has an apparent molecular mass of 160 kDa, appears on the surface of infected insect cells, and does not appear to be cleaved to glycoproteins gp 120 and gp41. Goats immunized with the 160-kDa protein have high titers of antibody that neutralizes virus infection as measured by viral gene expression or cell cytolysis. In addition, immune sera can block fusion of human immunodeficiency virus-infected cells in culture. Both neutralization and fusion-blocking activities are bound to and eluted from immobilized gp120.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.19.6924