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Humoral Immune Response to the Entire Human Immunodeficiency Virus Envelope Glycoprotein Made in Insect Cells
The human immunodeficiency virus envelope gene was expressed in insect cells by using a Baculovirus expression vector. The protein has an apparent molecular mass of 160 kDa, appears on the surface of infected insect cells, and does not appear to be cleaved to glycoproteins gp 120 and gp41. Goats imm...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 1987-10, Vol.84 (19), p.6924-6928 |
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| Main Authors: | , , , , , , , , , , , |
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| Language: | English |
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| container_end_page | 6928 |
| container_issue | 19 |
| container_start_page | 6924 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 84 |
| creator | Rusche, James R. Lynn, Debra L. Robert-Guroff, Marjorie Langlois, Alphonse J. Lyerly, H. Kim Carson, Helen Krohn, Kai Ranki, Annamari Gallo, Robert C. Bolognesi, Dani P. Putney, Scott D. Matthews, Thomas J. |
| description | The human immunodeficiency virus envelope gene was expressed in insect cells by using a Baculovirus expression vector. The protein has an apparent molecular mass of 160 kDa, appears on the surface of infected insect cells, and does not appear to be cleaved to glycoproteins gp 120 and gp41. Goats immunized with the 160-kDa protein have high titers of antibody that neutralizes virus infection as measured by viral gene expression or cell cytolysis. In addition, immune sera can block fusion of human immunodeficiency virus-infected cells in culture. Both neutralization and fusion-blocking activities are bound to and eluted from immobilized gp120. |
| doi_str_mv | 10.1073/pnas.84.19.6924 |
| format | article |
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Kim ; Carson, Helen ; Krohn, Kai ; Ranki, Annamari ; Gallo, Robert C. ; Bolognesi, Dani P. ; Putney, Scott D. ; Matthews, Thomas J.</creator><creatorcontrib>Rusche, James R. ; Lynn, Debra L. ; Robert-Guroff, Marjorie ; Langlois, Alphonse J. ; Lyerly, H. Kim ; Carson, Helen ; Krohn, Kai ; Ranki, Annamari ; Gallo, Robert C. ; Bolognesi, Dani P. ; Putney, Scott D. ; Matthews, Thomas J.</creatorcontrib><description>The human immunodeficiency virus envelope gene was expressed in insect cells by using a Baculovirus expression vector. The protein has an apparent molecular mass of 160 kDa, appears on the surface of infected insect cells, and does not appear to be cleaved to glycoproteins gp 120 and gp41. Goats immunized with the 160-kDa protein have high titers of antibody that neutralizes virus infection as measured by viral gene expression or cell cytolysis. In addition, immune sera can block fusion of human immunodeficiency virus-infected cells in culture. Both neutralization and fusion-blocking activities are bound to and eluted from immobilized gp120.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.84.19.6924</identifier><identifier>PMID: 3477816</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>AIDS/HIV ; Animals ; Antibody Formation ; Antiserum ; Baculoviridae ; baculovirus ; Biological and medical sciences ; Biotechnology ; Cell Line ; Cell lines ; cloning vectors ; envelopes ; Fundamental and applied biological sciences. Psychology ; Gels ; Genes ; Genes, Viral ; Genetic engineering ; Genetic technics ; Genetic Vectors ; glycoproteins ; Goats - immunology ; HIV ; HIV - genetics ; HIV - immunology ; human immunodeficiency virus ; immune response (humoral) ; Infections ; Insect genetics ; Insect vectors ; Insect Viruses - genetics ; Methods. Procedures. 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Kim</creatorcontrib><creatorcontrib>Carson, Helen</creatorcontrib><creatorcontrib>Krohn, Kai</creatorcontrib><creatorcontrib>Ranki, Annamari</creatorcontrib><creatorcontrib>Gallo, Robert C.</creatorcontrib><creatorcontrib>Bolognesi, Dani P.</creatorcontrib><creatorcontrib>Putney, Scott D.</creatorcontrib><creatorcontrib>Matthews, Thomas J.</creatorcontrib><title>Humoral Immune Response to the Entire Human Immunodeficiency Virus Envelope Glycoprotein Made in Insect Cells</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The human immunodeficiency virus envelope gene was expressed in insect cells by using a Baculovirus expression vector. The protein has an apparent molecular mass of 160 kDa, appears on the surface of infected insect cells, and does not appear to be cleaved to glycoproteins gp 120 and gp41. Goats immunized with the 160-kDa protein have high titers of antibody that neutralizes virus infection as measured by viral gene expression or cell cytolysis. In addition, immune sera can block fusion of human immunodeficiency virus-infected cells in culture. Both neutralization and fusion-blocking activities are bound to and eluted from immobilized gp120.</description><subject>AIDS/HIV</subject><subject>Animals</subject><subject>Antibody Formation</subject><subject>Antiserum</subject><subject>Baculoviridae</subject><subject>baculovirus</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>cloning vectors</subject><subject>envelopes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Genes</subject><subject>Genes, Viral</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors</subject><subject>glycoproteins</subject><subject>Goats - immunology</subject><subject>HIV</subject><subject>HIV - genetics</subject><subject>HIV - immunology</subject><subject>human immunodeficiency virus</subject><subject>immune response (humoral)</subject><subject>Infections</subject><subject>Insect genetics</subject><subject>Insect vectors</subject><subject>Insect Viruses - genetics</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>Molecular cloning</subject><subject>Moths</subject><subject>Recombinant Proteins - immunology</subject><subject>Spodoptera frugiperda</subject><subject>vaccination</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Virology</subject><subject>Viruses</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNqFkUtv1DAUhSMEKtPCGgkJ5EUFq0z9Gj8WLNCotCMVISFga3mcG5oqsYPtVMy_x6MJA93A6i7Od47v9amqFwQvCZbsYvQ2LRVfEr0UmvJH1YJgTWrBNX5cLTCmslac8qfVaUp3GGO9UvikOmFcSkXEohqupyFE26PNMEwe0GdIY_AJUA4o3wK69LmLgApl_YEJDbSd68C7HfrWxSkV5h76MAK66ncujDFk6Dz6aBtAZW5KmstoDX2fnlVPWtsneD7Ps-rrh8sv6-v65tPVZv3-pnaCs1zTlePbljHbtqLVTMgVt6zhW2oJboSz2LUAGjOFKRDRgFphha2SCjhIqTk7q94dcsdpO0DjwOdyoxljN9i4M8F25qHiu1vzPdwbqjXRsvjfzP4YfkyQshm65MoF1kOYklEEc6o5-S9Iyj-zldQFvDiALoaUIrTHZQg2-ybNvkmjuCHa7Jssjld_33Dk5-qKfj7rNjnbt9F616UjJpmmgtKCvZ6xff5v9cE7b_8JmHbq-ww_cyFfHsi7lEP8sxAWmrFff4XJ9A</recordid><startdate>19871001</startdate><enddate>19871001</enddate><creator>Rusche, James R.</creator><creator>Lynn, Debra L.</creator><creator>Robert-Guroff, Marjorie</creator><creator>Langlois, Alphonse J.</creator><creator>Lyerly, H. Kim</creator><creator>Carson, Helen</creator><creator>Krohn, Kai</creator><creator>Ranki, Annamari</creator><creator>Gallo, Robert C.</creator><creator>Bolognesi, Dani P.</creator><creator>Putney, Scott D.</creator><creator>Matthews, Thomas J.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19871001</creationdate><title>Humoral Immune Response to the Entire Human Immunodeficiency Virus Envelope Glycoprotein Made in Insect Cells</title><author>Rusche, James R. ; Lynn, Debra L. ; Robert-Guroff, Marjorie ; Langlois, Alphonse J. ; Lyerly, H. Kim ; Carson, Helen ; Krohn, Kai ; Ranki, Annamari ; Gallo, Robert C. ; Bolognesi, Dani P. ; Putney, Scott D. ; Matthews, Thomas J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c643t-25c4bf33aff6f936754a3d4b2a10d6ca0cfee903802e16de85080a878e4e77943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>AIDS/HIV</topic><topic>Animals</topic><topic>Antibody Formation</topic><topic>Antiserum</topic><topic>Baculoviridae</topic><topic>baculovirus</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>cloning vectors</topic><topic>envelopes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Genes</topic><topic>Genes, Viral</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors</topic><topic>glycoproteins</topic><topic>Goats - immunology</topic><topic>HIV</topic><topic>HIV - genetics</topic><topic>HIV - immunology</topic><topic>human immunodeficiency virus</topic><topic>immune response (humoral)</topic><topic>Infections</topic><topic>Insect genetics</topic><topic>Insect vectors</topic><topic>Insect Viruses - genetics</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>Molecular cloning</topic><topic>Moths</topic><topic>Recombinant Proteins - immunology</topic><topic>Spodoptera frugiperda</topic><topic>vaccination</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rusche, James R.</creatorcontrib><creatorcontrib>Lynn, Debra L.</creatorcontrib><creatorcontrib>Robert-Guroff, Marjorie</creatorcontrib><creatorcontrib>Langlois, Alphonse J.</creatorcontrib><creatorcontrib>Lyerly, H. 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Kim</au><au>Carson, Helen</au><au>Krohn, Kai</au><au>Ranki, Annamari</au><au>Gallo, Robert C.</au><au>Bolognesi, Dani P.</au><au>Putney, Scott D.</au><au>Matthews, Thomas J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Humoral Immune Response to the Entire Human Immunodeficiency Virus Envelope Glycoprotein Made in Insect Cells</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1987-10-01</date><risdate>1987</risdate><volume>84</volume><issue>19</issue><spage>6924</spage><epage>6928</epage><pages>6924-6928</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The human immunodeficiency virus envelope gene was expressed in insect cells by using a Baculovirus expression vector. The protein has an apparent molecular mass of 160 kDa, appears on the surface of infected insect cells, and does not appear to be cleaved to glycoproteins gp 120 and gp41. Goats immunized with the 160-kDa protein have high titers of antibody that neutralizes virus infection as measured by viral gene expression or cell cytolysis. In addition, immune sera can block fusion of human immunodeficiency virus-infected cells in culture. Both neutralization and fusion-blocking activities are bound to and eluted from immobilized gp120.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3477816</pmid><doi>10.1073/pnas.84.19.6924</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1987-10, Vol.84 (19), p.6924-6928 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_7392622 |
| source | JSTOR Archival Journals and Primary Sources Collection; PubMed Central |
| subjects | AIDS/HIV Animals Antibody Formation Antiserum Baculoviridae baculovirus Biological and medical sciences Biotechnology Cell Line Cell lines cloning vectors envelopes Fundamental and applied biological sciences. Psychology Gels Genes Genes, Viral Genetic engineering Genetic technics Genetic Vectors glycoproteins Goats - immunology HIV HIV - genetics HIV - immunology human immunodeficiency virus immune response (humoral) Infections Insect genetics Insect vectors Insect Viruses - genetics Methods. Procedures. Technologies Microbiology Molecular cloning Moths Recombinant Proteins - immunology Spodoptera frugiperda vaccination Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Viral Envelope Proteins - immunology Virology Viruses |
| title | Humoral Immune Response to the Entire Human Immunodeficiency Virus Envelope Glycoprotein Made in Insect Cells |
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