Genomic Sequence of hst, a Transforming Gene Encoding a Protein Homologous to Fibroblast Growth Factors and the int-2-Encoded Protein

hst is a transforming gene first identified from transformed NIH 3T3 cells that were transfected with DNA of a human stomach cancer. A genomic fragment of hst obtained directly from a human genomic library also has transforming activity. This fragment has a coding sequence identical to that of the h...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-10, Vol.84 (20), p.7305-7309
Main Authors: Yoshida, Teruhiko, Miyagawa, Kiyoshi, Odagiri, Hiroki, Sakamoto, Hiromi, Peter F. R. Little, Terada, Masaaki, Sugimura, Takashi
Format: Article
Language:English
Subjects:
3T3 cells
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Biological and medical sciences
carcinogenesis
Cell Transformation, Neoplastic - genetics
Complementary DNA
DNA
DNA, Neoplasm - genetics
Fibroblast Growth Factor 4
Fibroblast growth factors
Fibroblast Growth Factors - genetics
Fibroblasts - analysis
Fundamental and applied biological sciences. Psychology
genes
Genes. Genome
Genomics
growth factors
Growth Substances - genetics
man
Mice
Mice, Inbred BALB C
Mice, Nude
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Neoplasms, Experimental - etiology
NIH 3T3 cells
nucleotide sequence
Oncogene Proteins - genetics
Oncogenes
Open reading frames
Proto-Oncogene Proteins
Sequence Homology, Nucleic Acid
Stomach Neoplasms - genetics
TATA box
Citations: Items that cite this one
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Description
Summary:hst is a transforming gene first identified from transformed NIH 3T3 cells that were transfected with DNA of a human stomach cancer. A genomic fragment of hst obtained directly from a human genomic library also has transforming activity. This fragment has a coding sequence identical to that of the hst cDNA prepared from an NIH 3T3 transformant induced by DNA from a stomach cancer. The deduced amino acid sequence of the hst protein is 43%, 38%, and 40% homologous, respectively, to human basic fibroblast growth factor, human acidic fibroblast growth factor, and mouse int-2 protein in selected regions. This suggests that hst encodes a protein related to fibroblast growth factors, which are wide-spectrum mitogens, and to the int-2 protein, a potential oncogene product implicated in murine mammary carcinogenesis.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.20.7305