Model for Intermediate Steps in Monocytic Differentiation: c-myc, c-fms, and Ferritin as Markers

A mature hematopoietic cell represents the end product of a stepwise differentiation process. As a model system for studying differentiation, the human promyelocytic leukemia cell line HL-60 undergoes terminal monocytic/macrophagic differentiation following exposure to either phorbol 12-myristate 13...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-11, Vol.84 (21), p.7619-7623
Main Authors: Cayre, Yvon, Raynal, Marie-Cecile, Darzynkiewicz, Zbigniew, Dorner, Marianne H.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Biological and medical sciences
Cell adhesion
Cell cycle
Cell Differentiation
Cell differentiation, maturation, development, hematopoiesis
Cell growth
Cell Line
Cell lines
Cell physiology
Cellular differentiation
Cultured cells
Ferritins
Ferritins - analysis
Flow Cytometry
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Genetic Variation
Hematopoietic stem cells
Humans
Leukemia, Myeloid, Acute
Messenger RNA
Models, Biological
Molecular and cellular biology
Monocytes - cytology
Proto-Oncogenes
RNA
RNA, Messenger - genetics
Transcription, Genetic
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A mature hematopoietic cell represents the end product of a stepwise differentiation process. As a model system for studying differentiation, the human promyelocytic leukemia cell line HL-60 undergoes terminal monocytic/macrophagic differentiation following exposure to either phorbol 12-myristate 13-acetate or 1,25-dihydroxyvitamin D3. We have derived and analyzed a variant HL-60 cell line, 1F10, that permits the study of several intermediate steps in the myeloid differentiation process. These intermediate steps are documented by cell cycle data and phenotype analysis as well as markers such as c-myc, c-fms, and both subunits of ferritin.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.21.7619