Isolation and partial characterization of follistatin: a single-chain Mr 35,000 monomeric protein that inhibits the release of follicle-stimulating hormone

A Mr 35,000 protein with follicle-stimulating hormone release-inhibitory activity was isolated from porcine ovarian follicular fluid by heparin-Sepharose affinity chromatography, gel filtration on Sephacryl S-200, and multiple steps of high-performance liquid chromatography. The isolated molecule is...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-12, Vol.84 (23), p.8282-8286
Main Authors: Ueno, N, Ling, N, Ying, S.Y, Esch, F, Shimasaki, S, Guillemin, R
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids - analysis
Animals
BIOASSAY
Biochemistry
Biological and medical sciences
BODY FLUIDS
Cell physiology
CERDO
Chromatography, High Pressure Liquid
ENSAYO BIOLOGICO
ESSAI BIOLOG
Female
Follicle Stimulating Hormone - metabolism
Follicular fluid
Follistatin
Fundamental and applied biological sciences. Psychology
Gels
Glycoproteins - isolation & purification
Glycoproteins - physiology
GONADOTROPHINE
GONADOTROPINAS
GONADOTROPINS
Hormonal regulation
Hormones
ISOLATION TECHNIQUE
Liquid chromatography
LIQUIDE BIOLOGIQUE
LIQUIDOS CORPORALES
Molecular and cellular biology
Molecular Weight
OVAIRE
Ovarian Follicle - analysis
OVARIES
OVARIOS
Phosphates
PORCIN
PROTEINAS
PROTEINE
PROTEINS
Secretion
Solvents
SWINE
TECHNIQUE D2015E L'ISOLEMENT
TECNICAS DE AISLAMIENTO
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Description
Summary:A Mr 35,000 protein with follicle-stimulating hormone release-inhibitory activity was isolated from porcine ovarian follicular fluid by heparin-Sepharose affinity chromatography, gel filtration on Sephacryl S-200, and multiple steps of high-performance liquid chromatography. The isolated molecule is highly enriched in cysteines and is composed of a single polypeptide chain. In addition, it has no sequence homology with the previously characterized follicular fluid inhibins, which are heterodimeric proteins of Mr 32,000 with follicle-stimulating hormone release-inhibiting activity. This protein specifically inhibits the basal secretion of follicle-stimulating hormone, but not that of luteinizing hormone, in the rat anterior pituitary monolayer culture system with a halfmaximal effective dose of 2.5-6.0 ng/ml. Another form of the molecule of Mr 32,000 present in much lower concentration in follicular fluid was also isolated. It may differ from the Mr 35,000 form in glycosylation or carboxyl-terminal truncation. We suggest that this compound be called ``follistatin'' to signify its structural difference from inhibin.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.23.8282