Stable sodium sulfate tolerance in Brassica napus cv. Westar callus cultures

• Published in: In vitro cellular & developmental biology 1987-10, Vol.23 (10), p.713-716
• Main Authors: Chandler, S.F, Reid, D.M, Thorpe, T.A
• Format: Article
• Language: English
• Subjects: Adaptation to environment and cultivation conditions; Agronomy. Soil science and plant productions; Biological and medical sciences; Biotechnology; BRASSICA NAPUS; CAL; CALLO; CALLUS; CELL CULTURE; Cell lines; CHEMICOPHYSICAL PROPERTIES; Classical genetics, quantitative genetics, hybrids; CULTIVO DE CELULAS; CULTURE DE CELLULES; CULTURE MEDIA; Cultured cells; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; Genetics of eukaryotes. Biological and molecular evolution; MEDIO DE CULTIVO; Methods. Procedures. Technologies; MILIEU DE CULTURE; Miscellaneous; Plant cells and fungal cells; Plant Cellular and Developmental Biology; Plants; PROPIEDADES FISICO-QUIMICAS; PROPRIETE PHYSICO-CHIMIQUE; Pteridophyta, spermatophyta; SALT TOLERANCE; SODIO; SODIUM; Subcultures; Sugars; SULFATE; Sulfates; SULFATOS; SULPHATES; Table salt; TOLERANCE AU SEL; TOLERANCIA A LA SAL; Varietal selection. Specialized plant breeding, plant breeding aims; Vegetals
• ISSN: 0883-8364; 2327-431X
• DOI: 10.1007/BF02620985

Sodium-sulfate-tolerant callus of Brassica napus cv. Westar, selected on medium containing 105 mM$Na_{2}SO_4$, was maintained on medium without the salt to test for stability of tolerance. Tolerance to$Na_{2}SO_4$was retained even after 18 subcultures on no salt. This tissue also showed tolerance to$K_{2}SO_4$, NaCl, and KCl. However, with the exception of callus grown on KCl fresh weight yields were less than that of tolerant callus maintained continuously on$Na_{2}SO_4$. Tolerant callus maintained on no salt had a mixture of the compact morphology of unselected callus and the friable morphology of tolerant callus. Both callus types expressed salt tolerance. Sucrose, reducing sugars and proline concentrations were measured in unselected callus, tolerant callus maintained continuously on$Na_{2}SO_4$, and tolerant callus maintained on no salt. Sucrose levels were similar in all cases. Whether maintained on or off$Na_{2}SO_4$, tolerant callus had reducing sugars levels three to four times greater than unselected callus. Tolerant callus maintained on no salt had twice the amount of proline found in the unselected callus. Tolerant callus maintained in the absence of salt had an ash content, sodium concentration, and potassium concentration significantly lower than that of unselected callus.