Somatostatin and α2‐adrenergic agonists selectively inhibit vasopressin‐induced cyclic AMP accumulation in MDCK cells

The effect of somatostatin and α2‐adrenergic agonists on cyclic AMP accumulation was examined in MDCK cells, grown in denned medium. These hormones inhibited vasopressin‐induced cyclic AMP formation, without affecting either the basal or the glucagon‐ and prostaglandin E2‐stimulated level. Pretreati...

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Bibliographic Details
Published in:FEBS letters 1986-03, Vol.198 (1), p.38-42
Main Authors: Friedlander, Gerard, Amiel, Claude
Format: Article
Language:English
Subjects:
3-isobutyl-1-methylxanthine
Biological and medical sciences
bovine serum albumin
BSA
Cell physiology
cyclic AMP
Fundamental and applied biological sciences. Psychology
Hanks' balanced salt solution
HBSS
Hormonal regulation
IAP
IBMX
islet-activating protein (pertussis toxin)
Madin-Darby canine kidney
MDCK
MDCK cell
Molecular and cellular biology
Norepinephrine
Pertussis toxin
Somatostatin
somatotropin release-inhibiting factor (somatostatin)
SRIF
Vasopressin
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Summary:The effect of somatostatin and α2‐adrenergic agonists on cyclic AMP accumulation was examined in MDCK cells, grown in denned medium. These hormones inhibited vasopressin‐induced cyclic AMP formation, without affecting either the basal or the glucagon‐ and prostaglandin E2‐stimulated level. Pretreating the cells with pertussis toxin, or incubating them with MnCl2 at a low concentration reversed the effect of somatostatin and α2‐agonists. These results suggest that somatostatin and norepinephrine could selectively modulate the renal effect of vasopressin, via the inhibitory regulatory subunit (Ni) of adenylate cyclase.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(86)81180-2