Electrogenic reduction of the secondary quinone acceptor in chromatophores of Rhodospirillum rubrum

Electron transfer QA → QB has been reconstituted with added Q‐10 in Rhodospirillum rubrum chromatophores associated with a phospholipid‐impregnated collodion film. Rapid kinetics measurements of laser flash‐induced ΔΨ generated in the chromatophores show that whereas electron transfer from Qa − to Q...

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Bibliographic Details
Published in:FEBS letters 1986-07, Vol.202 (2), p.224-228
Main Authors: Kaminskaya, Olga P., Drachev, Lel A., Konstantinov, Alexander A., Semenov, Alexey Yu, Skulachev, Vladimir P.
Format: Article
Language:English
Subjects:
Binary oscillation
Biological and medical sciences
Cell structures and functions
Chloroplast, photosynthetic membrane and photosynthesis
Chromatophore
Fundamental and applied biological sciences. Psychology
Kinetics
Membrane potential
Molecular and cellular biology
Quinone acceptor
Rhodospirillum rubrum
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Summary:Electron transfer QA → QB has been reconstituted with added Q‐10 in Rhodospirillum rubrum chromatophores associated with a phospholipid‐impregnated collodion film. Rapid kinetics measurements of laser flash‐induced ΔΨ generated in the chromatophores show that whereas electron transfer from Qa − to QB upon the first flash is not electrogenic in dark‐adapted chromatophores, reduction of Q− B to Qbh2 induced by the second flash gives rise to an electrogenic phase with τ = 250 μs at pH 7.5 which contributes about 10% to the total ΔΨ generated upon the flash. The electrogenic phase is ascribed to vectorial protonation of Q2− B.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(86)80691-3