A ‘capillary racetrack’ method for isolation of magnetotactic bacteria

A capillary tube was developed in which an inoculum of magnetotactic bacteria that contained only a few contaminants could be separated from crude sediment in a few minutes. Sterile fluid was placed on one side of a wetted cotton plug and sediment was placed on the other side. Magnetotactic bacteria...

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Bibliographic Details
Published in:FEMS microbiology letters 1987, Vol.45 (1), p.31-35
Main Authors: Wolfe, R.S., Thauer, R.K., Pfennig, N.
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Isolation method
Magnetic field
Magnetotactic bacterium
Microbial ecology
Motility
Various environments (extraatmospheric space, air, water)
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Description
Summary:A capillary tube was developed in which an inoculum of magnetotactic bacteria that contained only a few contaminants could be separated from crude sediment in a few minutes. Sterile fluid was placed on one side of a wetted cotton plug and sediment was placed on the other side. Magnetotactic bacteria migrated quickly through the cotton toward the south pole of a stirring-bar magnet placed at the closed end of the capillary. Protozoa and chemotactic bacteria were significantly delayed in passage through the cotton.
ISSN:0378-1097
1574-6968
DOI:10.1016/0378-1097(87)90039-5