A ‘capillary racetrack’ method for isolation of magnetotactic bacteria

A capillary tube was developed in which an inoculum of magnetotactic bacteria that contained only a few contaminants could be separated from crude sediment in a few minutes. Sterile fluid was placed on one side of a wetted cotton plug and sediment was placed on the other side. Magnetotactic bacteria...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 1987, Vol.45 (1), p.31-35
Main Authors: Wolfe, R.S., Thauer, R.K., Pfennig, N.
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Isolation method
Magnetic field
Magnetotactic bacterium
Microbial ecology
Motility
Various environments (extraatmospheric space, air, water)
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 35
container_issue 1
container_start_page 31
container_title FEMS microbiology letters
container_volume 45
creator Wolfe, R.S.
Thauer, R.K.
Pfennig, N.
description A capillary tube was developed in which an inoculum of magnetotactic bacteria that contained only a few contaminants could be separated from crude sediment in a few minutes. Sterile fluid was placed on one side of a wetted cotton plug and sediment was placed on the other side. Magnetotactic bacteria migrated quickly through the cotton toward the south pole of a stirring-bar magnet placed at the closed end of the capillary. Protozoa and chemotactic bacteria were significantly delayed in passage through the cotton.
doi_str_mv 10.1016/0378-1097(87)90039-5
format article
fullrecord <record><control><sourceid>elsevier_pasca</sourceid><recordid>TN_cdi_pascalfrancis_primary_8280294</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0378109787900395</els_id><sourcerecordid>0378109787900395</sourcerecordid><originalsourceid>FETCH-LOGICAL-e1985-d4d354412c0800dfb109f7ed74cbb6dbe139cfbf184ab01513fbbf5b96882bf63</originalsourceid><addsrcrecordid>eNo9kDtOAzEURS0EEuGzAwoXFFAMPI89Y7tBiiK-ikQDteUvGCYzkT1CossyYHtZCQ5BNO82R0_3HoROCFwQIO0lUC4qApKfCX4uAaismh00IQ1nVStbsYsm_8g-Osj5DQBYDe0EPUzxevVl9TJ2nU6fOGnrx3Le16tvvPDj6-BwGBKOeej0GIceDwEv9Evvx2HUdowWmxI-RX2E9oLusj_-y0P0fHP9NLur5o-397PpvPJEiqZyzNGGMVJbEAAumNIqcO84s8a0znhCpQ0mEMG0AdIQGowJjSk7RG1CSw_R6fbvUmeru5B0b2NWyxQXZYEStYBasoJdbTFfunxEn1S20ffWu5i8HZUboiKgNgLVxo7a2FGCq1-BqqE_2c9mog</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>A ‘capillary racetrack’ method for isolation of magnetotactic bacteria</title><source>Alma/SFX Local Collection</source><creator>Wolfe, R.S. ; Thauer, R.K. ; Pfennig, N.</creator><creatorcontrib>Wolfe, R.S. ; Thauer, R.K. ; Pfennig, N.</creatorcontrib><description>A capillary tube was developed in which an inoculum of magnetotactic bacteria that contained only a few contaminants could be separated from crude sediment in a few minutes. Sterile fluid was placed on one side of a wetted cotton plug and sediment was placed on the other side. Magnetotactic bacteria migrated quickly through the cotton toward the south pole of a stirring-bar magnet placed at the closed end of the capillary. Protozoa and chemotactic bacteria were significantly delayed in passage through the cotton.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/0378-1097(87)90039-5</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>Animal, plant and microbial ecology ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Isolation method ; Magnetic field ; Magnetotactic bacterium ; Microbial ecology ; Motility ; Various environments (extraatmospheric space, air, water)</subject><ispartof>FEMS microbiology letters, 1987, Vol.45 (1), p.31-35</ispartof><rights>1987</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=8280294$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolfe, R.S.</creatorcontrib><creatorcontrib>Thauer, R.K.</creatorcontrib><creatorcontrib>Pfennig, N.</creatorcontrib><title>A ‘capillary racetrack’ method for isolation of magnetotactic bacteria</title><title>FEMS microbiology letters</title><description>A capillary tube was developed in which an inoculum of magnetotactic bacteria that contained only a few contaminants could be separated from crude sediment in a few minutes. Sterile fluid was placed on one side of a wetted cotton plug and sediment was placed on the other side. Magnetotactic bacteria migrated quickly through the cotton toward the south pole of a stirring-bar magnet placed at the closed end of the capillary. Protozoa and chemotactic bacteria were significantly delayed in passage through the cotton.</description><subject>Animal, plant and microbial ecology</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isolation method</subject><subject>Magnetic field</subject><subject>Magnetotactic bacterium</subject><subject>Microbial ecology</subject><subject>Motility</subject><subject>Various environments (extraatmospheric space, air, water)</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNo9kDtOAzEURS0EEuGzAwoXFFAMPI89Y7tBiiK-ikQDteUvGCYzkT1CossyYHtZCQ5BNO82R0_3HoROCFwQIO0lUC4qApKfCX4uAaismh00IQ1nVStbsYsm_8g-Osj5DQBYDe0EPUzxevVl9TJ2nU6fOGnrx3Le16tvvPDj6-BwGBKOeej0GIceDwEv9Evvx2HUdowWmxI-RX2E9oLusj_-y0P0fHP9NLur5o-397PpvPJEiqZyzNGGMVJbEAAumNIqcO84s8a0znhCpQ0mEMG0AdIQGowJjSk7RG1CSw_R6fbvUmeru5B0b2NWyxQXZYEStYBasoJdbTFfunxEn1S20ffWu5i8HZUboiKgNgLVxo7a2FGCq1-BqqE_2c9mog</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>Wolfe, R.S.</creator><creator>Thauer, R.K.</creator><creator>Pfennig, N.</creator><general>Elsevier B.V</general><general>Blackwell</general><scope>IQODW</scope></search><sort><creationdate>1987</creationdate><title>A ‘capillary racetrack’ method for isolation of magnetotactic bacteria</title><author>Wolfe, R.S. ; Thauer, R.K. ; Pfennig, N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e1985-d4d354412c0800dfb109f7ed74cbb6dbe139cfbf184ab01513fbbf5b96882bf63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isolation method</topic><topic>Magnetic field</topic><topic>Magnetotactic bacterium</topic><topic>Microbial ecology</topic><topic>Motility</topic><topic>Various environments (extraatmospheric space, air, water)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolfe, R.S.</creatorcontrib><creatorcontrib>Thauer, R.K.</creatorcontrib><creatorcontrib>Pfennig, N.</creatorcontrib><collection>Pascal-Francis</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolfe, R.S.</au><au>Thauer, R.K.</au><au>Pfennig, N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A ‘capillary racetrack’ method for isolation of magnetotactic bacteria</atitle><jtitle>FEMS microbiology letters</jtitle><date>1987</date><risdate>1987</risdate><volume>45</volume><issue>1</issue><spage>31</spage><epage>35</epage><pages>31-35</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>A capillary tube was developed in which an inoculum of magnetotactic bacteria that contained only a few contaminants could be separated from crude sediment in a few minutes. Sterile fluid was placed on one side of a wetted cotton plug and sediment was placed on the other side. Magnetotactic bacteria migrated quickly through the cotton toward the south pole of a stirring-bar magnet placed at the closed end of the capillary. Protozoa and chemotactic bacteria were significantly delayed in passage through the cotton.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><doi>10.1016/0378-1097(87)90039-5</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0378-1097
ispartof FEMS microbiology letters, 1987, Vol.45 (1), p.31-35
issn 0378-1097
1574-6968
language eng
recordid cdi_pascalfrancis_primary_8280294
source Alma/SFX Local Collection
subjects Animal, plant and microbial ecology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Isolation method
Magnetic field
Magnetotactic bacterium
Microbial ecology
Motility
Various environments (extraatmospheric space, air, water)
title A ‘capillary racetrack’ method for isolation of magnetotactic bacteria
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T08%3A24%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-elsevier_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20%E2%80%98capillary%20racetrack%E2%80%99%20method%20for%20isolation%20of%20magnetotactic%20bacteria&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Wolfe,%20R.S.&rft.date=1987&rft.volume=45&rft.issue=1&rft.spage=31&rft.epage=35&rft.pages=31-35&rft.issn=0378-1097&rft.eissn=1574-6968&rft.coden=FMLED7&rft_id=info:doi/10.1016/0378-1097(87)90039-5&rft_dat=%3Celsevier_pasca%3E0378109787900395%3C/elsevier_pasca%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-e1985-d4d354412c0800dfb109f7ed74cbb6dbe139cfbf184ab01513fbbf5b96882bf63%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true