Inhibition of Development in Myxococcus xanthus by Monoclonal Antibody 1604

Monoclonal antibody (mAb) 1604 is directed against a cell surface antigen of Myxococcus xanthus. Purified antibody 1604 inhibited development of M. xanthus under conditions of submerged culture procedure otherwise leading to fruiting body formation. Intact molecules of mAb 1604, as well as its Fab f...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-07, Vol.84 (13), p.4505-4508
Main Authors: Gill, James S., Jarvis, Bruce W., Dworkin, Martin
Format: Article
Language:English
Subjects:
Aggregation
Antibodies
Antibodies, Bacterial - immunology
Antibodies, Monoclonal - immunology
Antigens, Bacterial - immunology
Antigens, Bacterial - physiology
Antigens, Surface - immunology
Antigens, Surface - physiology
Bacteriology
Biological and medical sciences
Cell Adhesion
Cell Movement
Developmental biology
Fruiting bodies
Fundamental and applied biological sciences. Psychology
Growth, nutrition, cell differenciation
Immunoglobulin Fab Fragments - immunology
Microbiology
Myxococcales - immunology
Myxococcales - physiology
Neurons
Plant antigens
Prokaryotic cells
Reproduction, Asexual
Somatic cells
Spores, Bacterial
Sporulation
Surface antigens
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Monoclonal antibody (mAb) 1604 is directed against a cell surface antigen of Myxococcus xanthus. Purified antibody 1604 inhibited development of M. xanthus under conditions of submerged culture procedure otherwise leading to fruiting body formation. Intact molecules of mAb 1604, as well as its Fab fragments, inhibited developmental aggregation, autolysis, fruiting body formation, and sporulation. The addition of relatively small amounts of antibody every 4 hr was much more effective than a single large dose given at the onset of development. The inhibitory action of mAb 1604 on development was reversible after prolonged incubation of the antibody with cells; this was probably due to proteolytic degradation of the antibody. The effect of mAb 1604 on submerged bacterial development was neutralized by affinity-purified 1604 cell surface antigen. Another antibody, mAb 2788, directed against an M. xanthus cell surface antigen, did not block development. These data suggest that 1604 cell surface antigen is involved in contact-mediated cell interactions in M. xanthus.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.13.4505