Intramolecular Recombination between Partially Homologous Sequences in Escherichia coli and Xenopus Laevis Oocytes

We describe a system to analyze the individual contribution of a single physical DNA end on intramolecular recombination between partially homologous sequences. We took advantage of this partial sequence divergence to measure the distance separating the DNA end from the final recombination event. We...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-09, Vol.84 (18), p.6496-6500
Main Authors: Abastado, Jean-Pierre, Darche, Sylvie, Godeau, François, Cami, Brigitte, Kourilsky, Philippe
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chromosome Mapping
Complementary DNA
DNA
DNA, Bacterial - genetics
Enzymes
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genic rearrangement. Recombination. Transposable element
Histograms
Homologous recombination
Insect colonies
Molecular and cellular biology
Molecular genetics
Oligonucleotide probes
Oocytes
Oocytes - physiology
Plasmids
Recombination, Genetic
Sequence homology
Sequence Homology, Nucleic Acid
Xenopus laevis - genetics
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Description
Summary:We describe a system to analyze the individual contribution of a single physical DNA end on intramolecular recombination between partially homologous sequences. We took advantage of this partial sequence divergence to measure the distance separating the DNA end from the final recombination event. We show that a single physical DNA end stimulates recombination when located in a region of homology. Recombination frequency decreases gradually with the distance from the DNA end. A recombinational hot spot is found at the end of the region of homology. A large insertion of unrelated DNA interferes asymmetrically with this process, suggesting that a recombinogenic signal propagates along the region of homology.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.18.6496