Experimental and theoretical dynamics of isoelectric focusing - Elucidation of a general separation mechanism

The transient states in isoelectric focusing were monitored using a potential gradient array detector. Electric field profiles are presented which show the formation of transient moving boundaries, as well as the approach of the steady-state distribution, during the focusing of two- and three-compon...

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Bibliographic Details
Published in:Journal of Chromatography 1986-01, Vol.351 (1), p.17-29
Main Authors: Thormann, W., Mosher, R. A., Bier, M.
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Inorganic And Physical Chemistry
Other chromatographic methods
Online Access:Get full text
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Summary:The transient states in isoelectric focusing were monitored using a potential gradient array detector. Electric field profiles are presented which show the formation of transient moving boundaries, as well as the approach of the steady-state distribution, during the focusing of two- and three-component systems. The results are completely consistent with corresponding computer-simulation data. The focusing process is comprised of two sequential phases, a relatively rapid separation phase and a much slower stabilizing phase. It was found that the second phase can last as much as 20 times longer than the separation of the constituents.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)83468-3