Monoclonal Antibody That Defines Human Myoepithelium

• Published in: Proceedings of the National Academy of Sciences - PNAS 1985-11, Vol.82 (21), p.7409-7413
• Main Authors: Dairkee, Shahnaz Hashmi, Blayney, Carlene, Smith, Helene S., Hackett, Adeline J.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Antibodies, immunoglobulins; Antibodies, Monoclonal - isolation & purification; Biological and medical sciences; Breast - immunology; Breast - ultrastructure; Breast Neoplasms - classification; Breast Neoplasms - immunology; Breasts; Carcinoma; Carcinoma - immunology; Carcinoma, Intraductal, Noninfiltrating - immunology; Cell Differentiation; Electrophoresis, Polyacrylamide Gel; Epithelium - immunology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Keratins; Keratins - immunology; Mammary glands; Mice; Mice, Inbred BALB C - immunology; Molecular immunology; Monoclonal antibodies; Neoplasm Proteins - immunology; Reactivity; Salivary glands; Salivary Glands - immunology; Salivary Glands - ultrastructure; Sweat glands; Sweat Glands - immunology; Sweat Glands - ultrastructure; Tumors
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.82.21.7409

We have isolated a mouse monoclonal antibody that, upon immunohistochemical localization in frozen sections, displays specificity for human myoepithelial cells in the resting mammary gland, sweat glands, and salivary glands. Furthermore, this antibody was strongly and homogeneously reactive with frozen sections of 3 of 60 breast carcinoma specimens. Using immunolocalization techniques in conjunction with polyacrylamide gel electrophoresis, we have determined that the reactivity of this monoclonal antibody is directed toward a 51,000-dalton keratin polypeptide. The potential uses of this antibody in the prognosis of human mammary carcinoma and in understanding the role of the myoepithelium in development and differentiation are discussed.