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Monoclonal Antibody That Defines Human Myoepithelium
We have isolated a mouse monoclonal antibody that, upon immunohistochemical localization in frozen sections, displays specificity for human myoepithelial cells in the resting mammary gland, sweat glands, and salivary glands. Furthermore, this antibody was strongly and homogeneously reactive with fro...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 1985-11, Vol.82 (21), p.7409-7413 |
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| Main Authors: | , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c490t-caeb33dbd755414a2505002b720d34e5b24741e7d8e48724e9602c9da0b04bbc3 |
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| container_end_page | 7413 |
| container_issue | 21 |
| container_start_page | 7409 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 82 |
| creator | Dairkee, Shahnaz Hashmi Blayney, Carlene Smith, Helene S. Hackett, Adeline J. |
| description | We have isolated a mouse monoclonal antibody that, upon immunohistochemical localization in frozen sections, displays specificity for human myoepithelial cells in the resting mammary gland, sweat glands, and salivary glands. Furthermore, this antibody was strongly and homogeneously reactive with frozen sections of 3 of 60 breast carcinoma specimens. Using immunolocalization techniques in conjunction with polyacrylamide gel electrophoresis, we have determined that the reactivity of this monoclonal antibody is directed toward a 51,000-dalton keratin polypeptide. The potential uses of this antibody in the prognosis of human mammary carcinoma and in understanding the role of the myoepithelium in development and differentiation are discussed. |
| doi_str_mv | 10.1073/pnas.82.21.7409 |
| format | article |
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Furthermore, this antibody was strongly and homogeneously reactive with frozen sections of 3 of 60 breast carcinoma specimens. Using immunolocalization techniques in conjunction with polyacrylamide gel electrophoresis, we have determined that the reactivity of this monoclonal antibody is directed toward a 51,000-dalton keratin polypeptide. The potential uses of this antibody in the prognosis of human mammary carcinoma and in understanding the role of the myoepithelium in development and differentiation are discussed.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.21.7409</identifier><identifier>PMID: 2414776</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Antibodies ; Antibodies, immunoglobulins ; Antibodies, Monoclonal - isolation & purification ; Biological and medical sciences ; Breast - immunology ; Breast - ultrastructure ; Breast Neoplasms - classification ; Breast Neoplasms - immunology ; Breasts ; Carcinoma ; Carcinoma - immunology ; Carcinoma, Intraductal, Noninfiltrating - immunology ; Cell Differentiation ; Electrophoresis, Polyacrylamide Gel ; Epithelium - immunology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Keratins ; Keratins - immunology ; Mammary glands ; Mice ; Mice, Inbred BALB C - immunology ; Molecular immunology ; Monoclonal antibodies ; Neoplasm Proteins - immunology ; Reactivity ; Salivary glands ; Salivary Glands - immunology ; Salivary Glands - ultrastructure ; Sweat glands ; Sweat Glands - immunology ; Sweat Glands - ultrastructure ; Tumors</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1985-11, Vol.82 (21), p.7409-7413</ispartof><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-caeb33dbd755414a2505002b720d34e5b24741e7d8e48724e9602c9da0b04bbc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/21.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26380$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26380$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774,58219,58452</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8557504$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2414776$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dairkee, Shahnaz Hashmi</creatorcontrib><creatorcontrib>Blayney, Carlene</creatorcontrib><creatorcontrib>Smith, Helene S.</creatorcontrib><creatorcontrib>Hackett, Adeline J.</creatorcontrib><title>Monoclonal Antibody That Defines Human Myoepithelium</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We have isolated a mouse monoclonal antibody that, upon immunohistochemical localization in frozen sections, displays specificity for human myoepithelial cells in the resting mammary gland, sweat glands, and salivary glands. Furthermore, this antibody was strongly and homogeneously reactive with frozen sections of 3 of 60 breast carcinoma specimens. Using immunolocalization techniques in conjunction with polyacrylamide gel electrophoresis, we have determined that the reactivity of this monoclonal antibody is directed toward a 51,000-dalton keratin polypeptide. The potential uses of this antibody in the prognosis of human mammary carcinoma and in understanding the role of the myoepithelium in development and differentiation are discussed.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, immunoglobulins</subject><subject>Antibodies, Monoclonal - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Breast - immunology</subject><subject>Breast - ultrastructure</subject><subject>Breast Neoplasms - classification</subject><subject>Breast Neoplasms - immunology</subject><subject>Breasts</subject><subject>Carcinoma</subject><subject>Carcinoma - immunology</subject><subject>Carcinoma, Intraductal, Noninfiltrating - immunology</subject><subject>Cell Differentiation</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Epithelium - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Keratins</subject><subject>Keratins - immunology</subject><subject>Mammary glands</subject><subject>Mice</subject><subject>Mice, Inbred BALB C - immunology</subject><subject>Molecular immunology</subject><subject>Monoclonal antibodies</subject><subject>Neoplasm Proteins - immunology</subject><subject>Reactivity</subject><subject>Salivary glands</subject><subject>Salivary Glands - immunology</subject><subject>Salivary Glands - ultrastructure</subject><subject>Sweat glands</subject><subject>Sweat Glands - immunology</subject><subject>Sweat Glands - ultrastructure</subject><subject>Tumors</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNp9kM1v1DAUxC0EKkvLGQkJlAOCU7bPX3Fy6KEqlFZqxaWcLcdxWFeOvcQOYv97HG0U6IXTO8xv3owGoTcYthgEPd97Fbc12RK8FQyaZ2iDocFlxRp4jjYARJQ1I-wlehXjIwA0vIYTdEIYZkJUG8Tugw_aBa9ccemTbUN3KB52KhWfTW-9icXNNChf3B-C2du0M85Owxl60SsXzevlnqLv118erm7Ku29fb68u70qd41OplWkp7dpOcJ7zFOHAc6NWEOgoM7wlTDBsRFcbVgvCTFMB0U2noAXWtpqeoovj3_3UDqbTxqdRObkf7aDGgwzKyqeKtzv5I_yStMGUs-z_uPjH8HMyMcnBRm2cU96EKUpRMUaoEBk8P4J6DDGOpl8zMMh5ZznvLGsiCZbzztnx7t9qK78Mm_UPi66iVq4fldc2rljNueAwN_y0YPP_VV1zZD85l8zvlMn3_yUz8PYIPMYUxr-FKloD_QO6IacZ</recordid><startdate>19851101</startdate><enddate>19851101</enddate><creator>Dairkee, Shahnaz Hashmi</creator><creator>Blayney, Carlene</creator><creator>Smith, Helene S.</creator><creator>Hackett, Adeline J.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19851101</creationdate><title>Monoclonal Antibody That Defines Human Myoepithelium</title><author>Dairkee, Shahnaz Hashmi ; Blayney, Carlene ; Smith, Helene S. ; Hackett, Adeline J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-caeb33dbd755414a2505002b720d34e5b24741e7d8e48724e9602c9da0b04bbc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, immunoglobulins</topic><topic>Antibodies, Monoclonal - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>Breast - immunology</topic><topic>Breast - ultrastructure</topic><topic>Breast Neoplasms - classification</topic><topic>Breast Neoplasms - immunology</topic><topic>Breasts</topic><topic>Carcinoma</topic><topic>Carcinoma - immunology</topic><topic>Carcinoma, Intraductal, Noninfiltrating - immunology</topic><topic>Cell Differentiation</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Epithelium - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Keratins</topic><topic>Keratins - immunology</topic><topic>Mammary glands</topic><topic>Mice</topic><topic>Mice, Inbred BALB C - immunology</topic><topic>Molecular immunology</topic><topic>Monoclonal antibodies</topic><topic>Neoplasm Proteins - immunology</topic><topic>Reactivity</topic><topic>Salivary glands</topic><topic>Salivary Glands - immunology</topic><topic>Salivary Glands - ultrastructure</topic><topic>Sweat glands</topic><topic>Sweat Glands - immunology</topic><topic>Sweat Glands - ultrastructure</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dairkee, Shahnaz Hashmi</creatorcontrib><creatorcontrib>Blayney, Carlene</creatorcontrib><creatorcontrib>Smith, Helene S.</creatorcontrib><creatorcontrib>Hackett, Adeline J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dairkee, Shahnaz Hashmi</au><au>Blayney, Carlene</au><au>Smith, Helene S.</au><au>Hackett, Adeline J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoclonal Antibody That Defines Human Myoepithelium</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-11-01</date><risdate>1985</risdate><volume>82</volume><issue>21</issue><spage>7409</spage><epage>7413</epage><pages>7409-7413</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>We have isolated a mouse monoclonal antibody that, upon immunohistochemical localization in frozen sections, displays specificity for human myoepithelial cells in the resting mammary gland, sweat glands, and salivary glands. Furthermore, this antibody was strongly and homogeneously reactive with frozen sections of 3 of 60 breast carcinoma specimens. Using immunolocalization techniques in conjunction with polyacrylamide gel electrophoresis, we have determined that the reactivity of this monoclonal antibody is directed toward a 51,000-dalton keratin polypeptide. The potential uses of this antibody in the prognosis of human mammary carcinoma and in understanding the role of the myoepithelium in development and differentiation are discussed.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2414776</pmid><doi>10.1073/pnas.82.21.7409</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1985-11, Vol.82 (21), p.7409-7413 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_8557504 |
| source | JSTOR Archival Journals and Primary Sources Collection; PubMed Central |
| subjects | Animals Antibodies Antibodies, immunoglobulins Antibodies, Monoclonal - isolation & purification Biological and medical sciences Breast - immunology Breast - ultrastructure Breast Neoplasms - classification Breast Neoplasms - immunology Breasts Carcinoma Carcinoma - immunology Carcinoma, Intraductal, Noninfiltrating - immunology Cell Differentiation Electrophoresis, Polyacrylamide Gel Epithelium - immunology Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Keratins Keratins - immunology Mammary glands Mice Mice, Inbred BALB C - immunology Molecular immunology Monoclonal antibodies Neoplasm Proteins - immunology Reactivity Salivary glands Salivary Glands - immunology Salivary Glands - ultrastructure Sweat glands Sweat Glands - immunology Sweat Glands - ultrastructure Tumors |
| title | Monoclonal Antibody That Defines Human Myoepithelium |
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