investigation of stomatal ionic relations using guard cell protoplasts. II. Osmotic relations of guard cell protoplasts in short and long-term incubation

Measurements were made of the volume changes exhibited by isolated guard cell protoplasts (GCPs) of Commelina communis L. when exposed to a range of concentrations of external osmotica. In short-term incubation, GCPs behaved as osmometers and showed rapid volume changes in response to changing exter...

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Bibliographic Details
Published in:Journal of experimental botany 1985-11, Vol.36 (172), p.1739-1748
Main Author: Clint, G.M
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell membranes
Commelina communis
Diameters
Fundamental and applied biological sciences. Psychology
Guard cells
ion transport
Mops
Osmosis
Physiological regulation
Plant physiology and development
Plants
Potassium
Protoplasts
Tissue cultures, protoplasts
Turgor pressure
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Summary:Measurements were made of the volume changes exhibited by isolated guard cell protoplasts (GCPs) of Commelina communis L. when exposed to a range of concentrations of external osmotica. In short-term incubation, GCPs behaved as osmometers and showed rapid volume changes in response to changing external osmotic pressure (Πo). In long-term incubation, GCPs prepared and incubated with added external KCl showed further slow changes in volume, in a manner suggesting that regulation of volume occurred. Protoplasts prepared and incubated without added external KCl had smaller volumes for a given value of Πo, and their ability to regulate volume in long-term incubation was reduced or absent. Treatment with fusicoccin caused an increase in both the volume and the K+ content of GCPs. The increase in volume continuing after the increase in K+ content had ceased, in a manner similar to that observed in walled guard cells in epidermal strips.
ISSN:0022-0957
1460-2431