Interaction of cotton tissue culture cells and Verticillium dahliae

• Published in: In vitro cellular & developmental biology 1985-12, Vol.21 (12), p.659-664
• Main Authors: Altman, D.W, Stipanovic, R.D, Mitten, D.M, Heinstein, P
• Format: Article
• Language: English
• Subjects: Agronomy; Agronomy. Soil science and plant productions; Aldehydes; Biological and medical sciences; Biotechnology; Callus; Cell culture techniques; Cell lines; Chemical suspensions; CULTIVO DE TEJIDOS; CULTURE DE TISSUS; Cultured cells; Embryos; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Generalities; Genetics and breeding of economic plants; GOSSYPIUM ARBOREUM; Haploidy, in vitro culture applications, somatic hybrids; HIDROCARBUROS; HYDROCARBONS; HYDROCARBURE; Industrial applications and implications. Economical aspects; Inoculation; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; Pest resistance; Phytopathology. Animal pests. Plant and forest protection; Plant breeding: fundamental aspects and methodology; Terpenoids; TISSUE CULTURE; Varietal selection. Specialized plant breeding, plant breeding aims; VERTICILLIUM
• ISSN: 0883-8364; 2327-431X

Elicitation of sesquiterpenoid aldehyde phytoalexins in Gossypium arboreum cell suspension cultures was confirmed by thin layer chromatography, high performance reverse phase liquid chromatography, and an aniline-reaction assay after inoculation with heat-treated conidia of Verticillium dahliae. A 2.3x mean increase in total terpenoids was observed. Component phytoalexins varied, with either hemigossypol and gossypol being detected or the O-methylated terpenoids hemigossypol-6-methyl ether and related compounds. Long-term Gossypium suspension cultures were mixoploid with an increase in chromosome number and mean DNA content. Addition of V. dahliae elicitor(s) to the medium for embryo-proliferating callus of G. hirsutum inhibited growth and embryo production with a linear correlation (r = -0.87; P