One Gene and One Pseudogene for the Cytokeratin Endo A

The recombinant cDNA RecXVI, which hybridizes to the endo A mRNA, detects two specific bands on a Southern blot of EcoRI-digested genomic mouse DNA. We have screened a mouse genomic library with this cDNA and isolated these two sequences. Endo A is encoded by a 7.5-kilobase gene and by a 1.6-kilobas...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1985-02, Vol.82 (4), p.1155-1159
Main Authors: Vasseur, Marc, Duprey, Philippe, Brulet, Philippe, Jacob, Francois
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Cell Line
Cell lines
Cloning, Molecular
Complementary DNA
DNA
DNA, Recombinant
Embryo, Mammalian - metabolism
Embryonic cells
Epithelial cells
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genes
Genes. Genome
Introns
Keratins - genetics
L cells
Messenger RNA
Mice
Molecular and cellular biology
Molecular genetics
Repetitive Sequences, Nucleic Acid
RNA
Transcription, Genetic
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Summary:The recombinant cDNA RecXVI, which hybridizes to the endo A mRNA, detects two specific bands on a Southern blot of EcoRI-digested genomic mouse DNA. We have screened a mouse genomic library with this cDNA and isolated these two sequences. Endo A is encoded by a 7.5-kilobase gene and by a 1.6-kilobase pseudogene devoid of introns. A repetitive sequence belonging to the B2 family is located in the third intron of the gene. We have observed that transcription of B2 sequences and of the endo A gene are mutually exclusive.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.82.4.1155