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Specificity Analysis of Mouse Monoclonal Antibodies Defining Cell Surface Antigens of Human Renal Cancer

Six mouse monoclonal antibodies (mAbs) defining separate systems of cell surface antigens of cultured human renal cancer were tested for reactivity with normal fetal and adult tissues and with neoplastic tissues. Five of the mAbs identified glycoproteins of Mr160,000 (designated S4), Mr140,000 (F23)...

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Published in:Proceedings of the National Academy of Sciences - PNAS 1985-05, Vol.82 (9), p.2955-2959
Main Authors: Finstad, Connie L., Cordon-Cardo, Carlos, Bander, Neil H., Whitmore, Willet F., Melamed, Myron R., Old, Lloyd J.
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container_title Proceedings of the National Academy of Sciences - PNAS
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creator Finstad, Connie L.
Cordon-Cardo, Carlos
Bander, Neil H.
Whitmore, Willet F.
Melamed, Myron R.
Old, Lloyd J.
description Six mouse monoclonal antibodies (mAbs) defining separate systems of cell surface antigens of cultured human renal cancer were tested for reactivity with normal fetal and adult tissues and with neoplastic tissues. Five of the mAbs identified glycoproteins of Mr160,000 (designated S4), Mr140,000 (F23), Mr120,000 (S23 and S27), and Mr115,000 (S22). The glycoprotein component of Mr120,000 has been shown recently to be the adenosine deaminase binding protein (ADA-BP) and mAbS23 and mAbS27 define two distinct epitopes on ADA-BP. S22 was not detected on any normal fetal or adult tissues but was found on a subset of renal cancers. S4, F23, S23, and S27 defined distinct domains of the nephron: glomerulus (S4), proximal tubules (S4, F23, S23, and S27), and portions of Henle's loop (S23 and S27). mAbS4 also reacted with the interstitial matrix in the renal medulla and of other tissues, and mAbF23 reacted with fetal and adult fibroblasts. The S23 epitope of ADA-BP was expressed by placental trophoblasts and epithelial cells of breast, prostate, lung, and colon, whereas the S27 epitope was detected on a more limited range of cell types (trophoblasts and prostate epithelium). A panel of 20 renal cell carcinomas was typed for expression of these antigens; 7 phenotypes could be distinguished, with the S4+/F23+/S23+/S27+/S22+ or -phenotype (15 cases) being most common. The other antigenic system, V1, identified a heat-stable antigen that was widely expressed on cultured cell types but showed a restricted pattern of reactivity in tissues. V1 expression was limited to the adrenal cortex, Leydig cells, and the theca of ovarian follicles, and to adrenal cortical carcinomas.
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Five of the mAbs identified glycoproteins of Mr160,000 (designated S4), Mr140,000 (F23), Mr120,000 (S23 and S27), and Mr115,000 (S22). The glycoprotein component of Mr120,000 has been shown recently to be the adenosine deaminase binding protein (ADA-BP) and mAbS23 and mAbS27 define two distinct epitopes on ADA-BP. S22 was not detected on any normal fetal or adult tissues but was found on a subset of renal cancers. S4, F23, S23, and S27 defined distinct domains of the nephron: glomerulus (S4), proximal tubules (S4, F23, S23, and S27), and portions of Henle's loop (S23 and S27). mAbS4 also reacted with the interstitial matrix in the renal medulla and of other tissues, and mAbF23 reacted with fetal and adult fibroblasts. The S23 epitope of ADA-BP was expressed by placental trophoblasts and epithelial cells of breast, prostate, lung, and colon, whereas the S27 epitope was detected on a more limited range of cell types (trophoblasts and prostate epithelium). A panel of 20 renal cell carcinomas was typed for expression of these antigens; 7 phenotypes could be distinguished, with the S4+/F23+/S23+/S27+/S22+ or -phenotype (15 cases) being most common. The other antigenic system, V1, identified a heat-stable antigen that was widely expressed on cultured cell types but showed a restricted pattern of reactivity in tissues. V1 expression was limited to the adrenal cortex, Leydig cells, and the theca of ovarian follicles, and to adrenal cortical carcinomas.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3857626</pmid><doi>10.1073/pnas.82.9.2955</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 0027-8424
ispartof Proceedings of the National Academy of Sciences - PNAS, 1985-05, Vol.82 (9), p.2955-2959
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subjects Animals
Antibodies, immunoglobulins
Antibodies, Monoclonal - immunology
Antibody Specificity
antigen (tumor-associated)
Antigens
Antigens, Neoplasm - immunology
Antigens, Surface - immunology
Biological and medical sciences
Cancer
carcinoma
Cell Line
cell surface
Cultured cells
Epitopes
Female
Fetus
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Glycoproteins - immunology
Humans
kidney
Kidney - immunology
Kidney Neoplasms - immunology
Kidneys
Male
Membrane Proteins - immunology
Mice
Molecular immunology
Molecular Weight
monoclonal antibodies
Neoplasm Proteins - immunology
Organ Specificity
Reactivity
Renal cell carcinoma
Tissue culture techniques
Tumors
title Specificity Analysis of Mouse Monoclonal Antibodies Defining Cell Surface Antigens of Human Renal Cancer
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