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Specificity Analysis of Mouse Monoclonal Antibodies Defining Cell Surface Antigens of Human Renal Cancer
Six mouse monoclonal antibodies (mAbs) defining separate systems of cell surface antigens of cultured human renal cancer were tested for reactivity with normal fetal and adult tissues and with neoplastic tissues. Five of the mAbs identified glycoproteins of Mr160,000 (designated S4), Mr140,000 (F23)...
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Published in: | Proceedings of the National Academy of Sciences - PNAS 1985-05, Vol.82 (9), p.2955-2959 |
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creator | Finstad, Connie L. Cordon-Cardo, Carlos Bander, Neil H. Whitmore, Willet F. Melamed, Myron R. Old, Lloyd J. |
description | Six mouse monoclonal antibodies (mAbs) defining separate systems of cell surface antigens of cultured human renal cancer were tested for reactivity with normal fetal and adult tissues and with neoplastic tissues. Five of the mAbs identified glycoproteins of Mr160,000 (designated S4), Mr140,000 (F23), Mr120,000 (S23 and S27), and Mr115,000 (S22). The glycoprotein component of Mr120,000 has been shown recently to be the adenosine deaminase binding protein (ADA-BP) and mAbS23 and mAbS27 define two distinct epitopes on ADA-BP. S22 was not detected on any normal fetal or adult tissues but was found on a subset of renal cancers. S4, F23, S23, and S27 defined distinct domains of the nephron: glomerulus (S4), proximal tubules (S4, F23, S23, and S27), and portions of Henle's loop (S23 and S27). mAbS4 also reacted with the interstitial matrix in the renal medulla and of other tissues, and mAbF23 reacted with fetal and adult fibroblasts. The S23 epitope of ADA-BP was expressed by placental trophoblasts and epithelial cells of breast, prostate, lung, and colon, whereas the S27 epitope was detected on a more limited range of cell types (trophoblasts and prostate epithelium). A panel of 20 renal cell carcinomas was typed for expression of these antigens; 7 phenotypes could be distinguished, with the S4+/F23+/S23+/S27+/S22+ or -phenotype (15 cases) being most common. The other antigenic system, V1, identified a heat-stable antigen that was widely expressed on cultured cell types but showed a restricted pattern of reactivity in tissues. V1 expression was limited to the adrenal cortex, Leydig cells, and the theca of ovarian follicles, and to adrenal cortical carcinomas. |
doi_str_mv | 10.1073/pnas.82.9.2955 |
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Five of the mAbs identified glycoproteins of Mr160,000 (designated S4), Mr140,000 (F23), Mr120,000 (S23 and S27), and Mr115,000 (S22). The glycoprotein component of Mr120,000 has been shown recently to be the adenosine deaminase binding protein (ADA-BP) and mAbS23 and mAbS27 define two distinct epitopes on ADA-BP. S22 was not detected on any normal fetal or adult tissues but was found on a subset of renal cancers. S4, F23, S23, and S27 defined distinct domains of the nephron: glomerulus (S4), proximal tubules (S4, F23, S23, and S27), and portions of Henle's loop (S23 and S27). mAbS4 also reacted with the interstitial matrix in the renal medulla and of other tissues, and mAbF23 reacted with fetal and adult fibroblasts. The S23 epitope of ADA-BP was expressed by placental trophoblasts and epithelial cells of breast, prostate, lung, and colon, whereas the S27 epitope was detected on a more limited range of cell types (trophoblasts and prostate epithelium). A panel of 20 renal cell carcinomas was typed for expression of these antigens; 7 phenotypes could be distinguished, with the S4+/F23+/S23+/S27+/S22+ or -phenotype (15 cases) being most common. The other antigenic system, V1, identified a heat-stable antigen that was widely expressed on cultured cell types but showed a restricted pattern of reactivity in tissues. V1 expression was limited to the adrenal cortex, Leydig cells, and the theca of ovarian follicles, and to adrenal cortical carcinomas.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.9.2955</identifier><identifier>PMID: 3857626</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Antibodies, immunoglobulins ; Antibodies, Monoclonal - immunology ; Antibody Specificity ; antigen (tumor-associated) ; Antigens ; Antigens, Neoplasm - immunology ; Antigens, Surface - immunology ; Biological and medical sciences ; Cancer ; carcinoma ; Cell Line ; cell surface ; Cultured cells ; Epitopes ; Female ; Fetus ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Glycoproteins - immunology ; Humans ; kidney ; Kidney - immunology ; Kidney Neoplasms - immunology ; Kidneys ; Male ; Membrane Proteins - immunology ; Mice ; Molecular immunology ; Molecular Weight ; monoclonal antibodies ; Neoplasm Proteins - immunology ; Organ Specificity ; Reactivity ; Renal cell carcinoma ; Tissue culture techniques ; Tumors</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1985-05, Vol.82 (9), p.2955-2959</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c611t-d2129f7355807259a15451a9bf5f8aac583d1e7997de99a2c71c1e1b71e73f923</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/9.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25297$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25297$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774,58219,58452</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9141990$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3857626$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Finstad, Connie L.</creatorcontrib><creatorcontrib>Cordon-Cardo, Carlos</creatorcontrib><creatorcontrib>Bander, Neil H.</creatorcontrib><creatorcontrib>Whitmore, Willet F.</creatorcontrib><creatorcontrib>Melamed, Myron R.</creatorcontrib><creatorcontrib>Old, Lloyd J.</creatorcontrib><title>Specificity Analysis of Mouse Monoclonal Antibodies Defining Cell Surface Antigens of Human Renal Cancer</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Six mouse monoclonal antibodies (mAbs) defining separate systems of cell surface antigens of cultured human renal cancer were tested for reactivity with normal fetal and adult tissues and with neoplastic tissues. Five of the mAbs identified glycoproteins of Mr160,000 (designated S4), Mr140,000 (F23), Mr120,000 (S23 and S27), and Mr115,000 (S22). The glycoprotein component of Mr120,000 has been shown recently to be the adenosine deaminase binding protein (ADA-BP) and mAbS23 and mAbS27 define two distinct epitopes on ADA-BP. S22 was not detected on any normal fetal or adult tissues but was found on a subset of renal cancers. S4, F23, S23, and S27 defined distinct domains of the nephron: glomerulus (S4), proximal tubules (S4, F23, S23, and S27), and portions of Henle's loop (S23 and S27). mAbS4 also reacted with the interstitial matrix in the renal medulla and of other tissues, and mAbF23 reacted with fetal and adult fibroblasts. The S23 epitope of ADA-BP was expressed by placental trophoblasts and epithelial cells of breast, prostate, lung, and colon, whereas the S27 epitope was detected on a more limited range of cell types (trophoblasts and prostate epithelium). A panel of 20 renal cell carcinomas was typed for expression of these antigens; 7 phenotypes could be distinguished, with the S4+/F23+/S23+/S27+/S22+ or -phenotype (15 cases) being most common. The other antigenic system, V1, identified a heat-stable antigen that was widely expressed on cultured cell types but showed a restricted pattern of reactivity in tissues. V1 expression was limited to the adrenal cortex, Leydig cells, and the theca of ovarian follicles, and to adrenal cortical carcinomas.</description><subject>Animals</subject><subject>Antibodies, immunoglobulins</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity</subject><subject>antigen (tumor-associated)</subject><subject>Antigens</subject><subject>Antigens, Neoplasm - immunology</subject><subject>Antigens, Surface - immunology</subject><subject>Biological and medical sciences</subject><subject>Cancer</subject><subject>carcinoma</subject><subject>Cell Line</subject><subject>cell surface</subject><subject>Cultured cells</subject><subject>Epitopes</subject><subject>Female</subject><subject>Fetus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Glycoproteins - immunology</subject><subject>Humans</subject><subject>kidney</subject><subject>Kidney - immunology</subject><subject>Kidney Neoplasms - immunology</subject><subject>Kidneys</subject><subject>Male</subject><subject>Membrane Proteins - immunology</subject><subject>Mice</subject><subject>Molecular immunology</subject><subject>Molecular Weight</subject><subject>monoclonal antibodies</subject><subject>Neoplasm Proteins - immunology</subject><subject>Organ Specificity</subject><subject>Reactivity</subject><subject>Renal cell carcinoma</subject><subject>Tissue culture techniques</subject><subject>Tumors</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNqFkc-L1DAUx4Mo67h69SAIPYi3jknaNH0HD8u4usKK4Oo5ZNKX2SydZExacf77TXfGMoLgJYH3_XzfT0JeMrpkVFbvdl6nZcuXsOQgxCOyYBRY2dRAH5MFpVyWbc3rp-RZSneUUhAtPSNnVStkw5sFub3ZoXHWGTfsiwuv-31yqQi2-BLGhPn1wfQhx7M4uHXoHKbiA1rnnd8UK-z74maMVht8ADboH9xX41b74htOxpX2BuNz8sTqPuGL439Ofny8_L66Kq-_fvq8urguTcPYUHaccbCyErlPyQVoJmrBNKytsK3WRrRVx1ACyA4BNDeSGYZsLXOwssCrc_L-kHc3rrfYGfRD1L3aRbfVca-CdupvxbtbtQm_VAWyaUX2vz36Y_g5YhrU1iWT59Qe80qUbChQVrX_BVnNpeBNncHlATQxpBTRzs0wqqYbqumGquUK1HTDbHh9OsKMH4-W9TdHXSejexvzgl2aMWA1A6AnDU7p_6hzGWXHvh_w93BS759g1l8d9Ls0hDgDXHCQ1T2MFsZH</recordid><startdate>19850501</startdate><enddate>19850501</enddate><creator>Finstad, Connie L.</creator><creator>Cordon-Cardo, Carlos</creator><creator>Bander, Neil H.</creator><creator>Whitmore, Willet F.</creator><creator>Melamed, Myron R.</creator><creator>Old, Lloyd J.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19850501</creationdate><title>Specificity Analysis of Mouse Monoclonal Antibodies Defining Cell Surface Antigens of Human Renal Cancer</title><author>Finstad, Connie L. ; Cordon-Cardo, Carlos ; Bander, Neil H. ; Whitmore, Willet F. ; Melamed, Myron R. ; Old, Lloyd J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c611t-d2129f7355807259a15451a9bf5f8aac583d1e7997de99a2c71c1e1b71e73f923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Antibodies, immunoglobulins</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibody Specificity</topic><topic>antigen (tumor-associated)</topic><topic>Antigens</topic><topic>Antigens, Neoplasm - immunology</topic><topic>Antigens, Surface - immunology</topic><topic>Biological and medical sciences</topic><topic>Cancer</topic><topic>carcinoma</topic><topic>Cell Line</topic><topic>cell surface</topic><topic>Cultured cells</topic><topic>Epitopes</topic><topic>Female</topic><topic>Fetus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Glycoproteins - immunology</topic><topic>Humans</topic><topic>kidney</topic><topic>Kidney - immunology</topic><topic>Kidney Neoplasms - immunology</topic><topic>Kidneys</topic><topic>Male</topic><topic>Membrane Proteins - immunology</topic><topic>Mice</topic><topic>Molecular immunology</topic><topic>Molecular Weight</topic><topic>monoclonal antibodies</topic><topic>Neoplasm Proteins - immunology</topic><topic>Organ Specificity</topic><topic>Reactivity</topic><topic>Renal cell carcinoma</topic><topic>Tissue culture techniques</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Finstad, Connie L.</creatorcontrib><creatorcontrib>Cordon-Cardo, Carlos</creatorcontrib><creatorcontrib>Bander, Neil H.</creatorcontrib><creatorcontrib>Whitmore, Willet F.</creatorcontrib><creatorcontrib>Melamed, Myron R.</creatorcontrib><creatorcontrib>Old, Lloyd J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Finstad, Connie L.</au><au>Cordon-Cardo, Carlos</au><au>Bander, Neil H.</au><au>Whitmore, Willet F.</au><au>Melamed, Myron R.</au><au>Old, Lloyd J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specificity Analysis of Mouse Monoclonal Antibodies Defining Cell Surface Antigens of Human Renal Cancer</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-05-01</date><risdate>1985</risdate><volume>82</volume><issue>9</issue><spage>2955</spage><epage>2959</epage><pages>2955-2959</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Six mouse monoclonal antibodies (mAbs) defining separate systems of cell surface antigens of cultured human renal cancer were tested for reactivity with normal fetal and adult tissues and with neoplastic tissues. Five of the mAbs identified glycoproteins of Mr160,000 (designated S4), Mr140,000 (F23), Mr120,000 (S23 and S27), and Mr115,000 (S22). The glycoprotein component of Mr120,000 has been shown recently to be the adenosine deaminase binding protein (ADA-BP) and mAbS23 and mAbS27 define two distinct epitopes on ADA-BP. S22 was not detected on any normal fetal or adult tissues but was found on a subset of renal cancers. S4, F23, S23, and S27 defined distinct domains of the nephron: glomerulus (S4), proximal tubules (S4, F23, S23, and S27), and portions of Henle's loop (S23 and S27). mAbS4 also reacted with the interstitial matrix in the renal medulla and of other tissues, and mAbF23 reacted with fetal and adult fibroblasts. The S23 epitope of ADA-BP was expressed by placental trophoblasts and epithelial cells of breast, prostate, lung, and colon, whereas the S27 epitope was detected on a more limited range of cell types (trophoblasts and prostate epithelium). A panel of 20 renal cell carcinomas was typed for expression of these antigens; 7 phenotypes could be distinguished, with the S4+/F23+/S23+/S27+/S22+ or -phenotype (15 cases) being most common. The other antigenic system, V1, identified a heat-stable antigen that was widely expressed on cultured cell types but showed a restricted pattern of reactivity in tissues. V1 expression was limited to the adrenal cortex, Leydig cells, and the theca of ovarian follicles, and to adrenal cortical carcinomas.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3857626</pmid><doi>10.1073/pnas.82.9.2955</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Antibodies, immunoglobulins Antibodies, Monoclonal - immunology Antibody Specificity antigen (tumor-associated) Antigens Antigens, Neoplasm - immunology Antigens, Surface - immunology Biological and medical sciences Cancer carcinoma Cell Line cell surface Cultured cells Epitopes Female Fetus Fundamental and applied biological sciences. Psychology Fundamental immunology Glycoproteins - immunology Humans kidney Kidney - immunology Kidney Neoplasms - immunology Kidneys Male Membrane Proteins - immunology Mice Molecular immunology Molecular Weight monoclonal antibodies Neoplasm Proteins - immunology Organ Specificity Reactivity Renal cell carcinoma Tissue culture techniques Tumors |
title | Specificity Analysis of Mouse Monoclonal Antibodies Defining Cell Surface Antigens of Human Renal Cancer |
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