Gas—liquid chromatographic determination of toloxatone in human plasma : Routine analysis of a wide range of drug concentrations using a nitrogen-selective detector

A selective and sensitive gas—liquid chromatographic (GLC) method has been developed for the measurement of toloxatone at therapeutic concentrations in plasma. The technique is based on a single extraction from plasma at pH 10, the preparation of a trimethylsilyl derivative and detection by a nitrog...

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Bibliographic Details
Published in:Journal of chromatography 1983-01, Vol.274, p.139-148
Main Authors: Vajta, S., Le Moing, J.P., Rovei, V.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Online Access:Get full text
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Summary:A selective and sensitive gas—liquid chromatographic (GLC) method has been developed for the measurement of toloxatone at therapeutic concentrations in plasma. The technique is based on a single extraction from plasma at pH 10, the preparation of a trimethylsilyl derivative and detection by a nitrogen-selective detector. The traditional calibration curve, peak-area ratio of toloxatone to internal standard versus toloxatone plasma concentration, was slightly concave for the wide concentration considered (10–3000 ng/ml). As a consequence, the linear least-squares regression analysis gave a negative intercept on the y-axis which affected quantitation accuracy of low plasma concentration values. A calibration method taking into consideration the non-linearity of the calibration curve is proposed. The characteristics of the detector were examined in order to analyse response linearity and sensitivity. A linear relationship was found between background current and detector sensitivity.
ISSN:0378-4347
0021-9673
DOI:10.1016/S0378-4347(00)84417-5