Physico-chemical factors governing partition behaviour of solutes and particles in aqueous polymeric biphasic systems : III. Features of solutes and biological particles detected by the partition technique

• Published in: Journal of Chromatography A 1983, Vol.256 (1), p.49-59
• Main Authors: Zaslavsky, B.Yu, Mestechkina, N.M., Miheeva, L.M., Rogozhin, S.V.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Molecular biophysics; Physico-chemical properties of biomolecules
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(01)88211-X

The partition behaviour of human serum albumin and oxyhaemoglobin and several amino acids and small peptides was studied in the aqueous Ficoll—dextran biphasic system as a function of the ionic composition and pH. The partition coefficients of the solutes were expressed in terms of the equivalent number of CH 2 groups, n CH 2 , and the equivalent number of carboxyl groups, m. The physical meaning of these two parameters and of the relationships found between them and pH for the proteins examined are discussed. A correlation was established between the difference in the relative hydrophobicities of the individual phases of various water—organic solvent systems and the interfacial tension, γ 12, of the systems. It is argued that a relation of a similar type exists for the aqueous polymeric biphasic systems. The possibility of estimating the relative intensity of Van der Waals and hydration interactions of a solute and particle surface by examination of their partitioning in a biphasic system calibrated for the hydrophobic and hydration properties of the phases is discussed.