ISOLATION AND CHARACTERIZATION OF SMALL PLASMIDS FROM RHIZOBIUM MELILOTI

Eight isolates of Rhizobium meliloti were obtained from the root nodules of Trigonella foenumgraecum (fenugreek). They were analysed for their plasmid content by agarose gel electrophoresis. Both large and small plasmids were observed. The presence of small plasmids (Molecular weight (Mr) less than...

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Bibliographic Details
Published in:Current science (Bangalore) 1984-02, Vol.53 (4), p.186-188
Main Authors: D'SOUZA, M. R., NOTANI, N. K.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Centrifugation
DNA probes
Economic plant physiology
Electrophoresis
Fenugreek
Fundamental and applied biological sciences. Psychology
Gels
Genetics
Mission oriented research
Molecular weight
Plasmids
Sinorhizobium meliloti
Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
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Summary:Eight isolates of Rhizobium meliloti were obtained from the root nodules of Trigonella foenumgraecum (fenugreek). They were analysed for their plasmid content by agarose gel electrophoresis. Both large and small plasmids were observed. The presence of small plasmids (Molecular weight (Mr) less than 10 × 106) is reported here for the first time. Small plasmids were detected in at least 3 strains of Rhizobium meliloti. The molecular weight of pRmD04b was estimated to be 5.2 × 106. Strain Rm01 has plasmids 2 smaller than 5.2 × 106 and Rm06 has one small plasmid a little bigger than 5.2 × 106. Large plasmids were detected in Rm01, 04, 06 and 30 which were a little larger than plasmid RP4 (Mr 34 × 106). Strain Rm10 harbours 2 large plasmids only which migrate much slower than RP4 through the agarose gel.
ISSN:0011-3891