Expression of human α1‐antitrypsin in Escherichia coli

Complementary DNA coding for human α1‐antitrypsin has been placed under the control of the λPR promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor), Escherichia coli cells harbouring the recombinant plas...

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Bibliographic Details
Published in:FEBS letters 1984-01, Vol.166 (1), p.67-70
Main Authors: Bollen, Alex, Loriau, Rosette, Herzog, Albert, Hérion, Pascal
Format: Article
Language:English
Subjects:
Bacterial expression of human cDNA
Biological and medical sciences
Biotechnology
Cloning of human cDNA
Fundamental and applied biological sciences. Psychology
Gene expression
Genetic engineering
Genetic technics
Human α1-antitrypsin
Immunoassay for α1-antitrypsin
Methods. Procedures. Technologies
Miscellaneous
Molecular and cellular biology
Molecular genetics
Plasma protein
Recombinant DNA
Synthetic digonucleotides and genes. Sequencing
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Summary:Complementary DNA coding for human α1‐antitrypsin has been placed under the control of the λPR promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor), Escherichia coli cells harbouring the recombinant plasmid pULB1114 express human α1‐antitrypsin (± 9000 molecules/cell). The product has a M r of 44 000, corresponding to mature unglycosylated α1‐antitrypsin.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(84)80046-0