Acetylcholine enzyme sensor for determining methamidophos insecticide: Evaluation of some genetically modified acetylcholinesterases from Drosophila melanogaster

A sensitive screen-printed amperometric sensor suitable for rapid determination of the concentration of the insecticide methamidophos was developed. It was based on the principle of inhibition of acetylcholinesterase (AChE) activity. The first part of the study was focused on the screening of severa...

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Bibliographic Details
Published in:Analytica chimica acta 2001-04, Vol.434 (1), p.1-8
Main Authors: Nunes, Gilvanda Silva, Montesinos, Thierry, Marques, Paulo Brasil O., Fournier, Didier, Marty, Jean Louis
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensors
Biotechnology
Fundamental and applied biological sciences. Psychology
Inhibition constant ( ki)
Methamidophos
Methods. Procedures. Technologies
Recombinant acetylcholinesterases
Screen-printed electrodes
Various methods and equipments
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Summary:A sensitive screen-printed amperometric sensor suitable for rapid determination of the concentration of the insecticide methamidophos was developed. It was based on the principle of inhibition of acetylcholinesterase (AChE) activity. The first part of the study was focused on the screening of several genetically modified AChEs in order to select the most sensitive enzyme towards the methamidophos. Values for the bimolecular constant k i were also determined. In a second part of the study, we compared the lowest detectable methamidophos concentrations using different immobilised AChEs. The lowest detectable concentration in a standard solution was 1.4 ppb for the AChE(Dros)-B03 mutant compared to 4.8 ppb for the wild type AChE and 53 ppb for the electric eel source.
ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(01)00795-4