Genetic variants in meiotic program initiation pathway genes are associated with spermatogenic impairment in a Han Chinese population

• Published in: PloS one 2013-01, Vol.8 (1), p.e53443
• Main Authors: Lu, Chuncheng, Xu, Miaofei, Wang, Ying, Qin, Yufeng, Du, Guizhen, Wu, Wei, Han, Xiumei, Ji, Chao, Yang, Yanli, Gu, Aihua, Xia, Yankai, Song, Ling, Wang, Shoulin, Wang, Xinru
• Format: Article
• Language: English
• Subjects: Acids; Adaptor Proteins, Signal Transducing; Adult; Apoptosis; Asian Continental Ancestry Group - genetics; Azoospermia - congenital; Biological effects; Biology; Case-Control Studies; Cell cycle; Centrifugation; China; Confidence intervals; Cytochrome; Cytochrome P-450; Cytochrome P-450 Enzyme System - genetics; Education; Epidemiology; Gene expression; Genes; Genetic diversity; Genetic research; Genetic variance; Genetic Variation - genetics; Humans; Impairment; In vivo methods and tests; Infertility; Infertility, Male - genetics; Infertility, Male - pathology; Laboratories; Male; Medicine; Meiosis; Meiosis - genetics; Metabolism; Nutrition; Oligospermia - genetics; Oligospermia - pathology; Oligozoospermia; Otolaryngology; Oxidation; Patients; Polymorphism, Single Nucleotide; Population studies; Proteins - genetics; Public health; Reproductive health; Reproductive technology; Retinoic Acid 4-Hydroxylase; Risk Factors; RNA-Binding Proteins - genetics; Rodents; Semen; Signal Transduction - genetics; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Sperm; Spermatogenesis; Spermatogenesis - genetics; Toxicology; Young Adult
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0053443

The meiotic program initiation pathway genes (CYP26B1, NANOS1 and STRA8) have been proposed to play key roles in spermatogenesis. To elucidate the exact role of the genetic variants of the meiosis initiation genes in spermatogenesis, we genotyped the potential functional genetic variants of CYP26B1, NANOS1 and STRA8 genes, and evaluated their effects on spermatogenesis in our study population. In this study, all subjects were volunteers from the affiliated hospitals of Nanjing Medical University between March 2004 and July 2009 (NJMU Infertile Study). Total 719 idiopathic infertile cases were recruited and divided into three groups according to WHO semen parameters: 201 azoospermia patients (no sperm in the ejaculate even after centrifugation), 155 oligozoospermia patients (sperm counts 20×10(6)/ml). The control group consisted of 383 subjects with normal semen parameters, all of which had fathered at least one child without assisted reproductive technologies. Eight single nucleotide polymorphisms (SNPs) in CYP26B1, NANOS1 and STRA8 genes were determined by TaqMan allelic discrimination assay in 719 idiopathic infertile men and 383 healthy controls. The genetic variant rs10269148 of STRA8 gene showed higher risk of spermatogenic impairment in the groups of abnormospermia (including azoospermia subgroup and oligozoospermia subgroup) and azoospermia than the controls with odds ratios and 95% confidence intervals of 2.52 (1.29-4.94) and 2.92 (1.41-6.06), respectively (P = 0.006, 0.002 respective). Notably, larger sample size studies and in vivo or in vitro functional studies are needed to substantiate the biological roles of these variants. Our results provided epidemiological evidence supporting the involvement of genetic polymorphisms of the meiotic program initiation genes in modifying the risk of azoospermia and oligozoospermia in a Han-Chinese population.